Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos

doi:10.1016/j.mrfmmm.2016.11.001

	Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos
	Zhou, Rong 1,2,3; Song, Jing'e 5; Si, Jing1,2,3 ; Zhang, Hong1,2,3 ; Liu, Bin 5; Gan, Lu1,2,3 ; Zhou, Xin1,2,3 ; Wang, Yupei 1,2,3,4; Yan, Junfang 1,2,3,4; Zhang, Qianjing 1,2,3,4
刊名	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
	2016-11-01
卷号	793 页码:41-50
关键词	CORM-3 Zebrafish embryos X-ray irradiation Carbon-ion irradiation Oxidative stress Apoptosis
ISSN号	0027-5107
DOI	10.1016/j.mrfmmm.2016.11.001
英文摘要	The inhibitory effects of carbon monoxide (CO), generated by Ru(CO)(3)Cl-glycinate [CO-releasing molecule (CORM-3)], on developmental toxicity in zebrafish embryos induced by ionizing radiation with different linear energy transfer (LET) were studied. Zebrafish embryos at 5 h post-fertilization were irradiated with X-ray (low-LET) and carbon-ion (high-LET) with or without pretreatment of CORM-3 1 h before irradiation. CORM-3 pre-treatment showed a significant inhibitory effect on X-ray irradiation-induced developmental toxicity, but had little effect on carbon-ion irradiation-induced developmental toxicity. X-ray irradiation-induced significant increase in ROS levels and cell apoptosis could be modified by CORM-3 pretreatment. However, embryos exposed to carbon-ion irradiation showed significantly increase of cell apoptosis without obvious ROS generation, which could not be attenuated by CORM-3 pretreatment. CORM-3 could inhibit apoptosis induced by ionizing radiation with low-LET as an effective ROS scavenger. The expression of pro-apoptotic genes increased significantly after X-ray irradiation, but increased expression was reduced markedly when CORM-3 was applied before irradiation. Moreover, the protein levels of P53 and,gamma-H2AX increased markedly after X-ray irradiation, which could be modified by the presence of CORM-3. The protective effect of CORM-3 on X-ray irradiation occurred mainly by suppressing ROS generation and DNA damage, and thus inhibiting the activation of P53 and the mitochondria) apoptotic pathway, leading to the attenuation of cell apoptosis and consequently alleviating X-ray irradiation-induced developmental toxicity at lethal and sub-lethal levels. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	Key Program of National Natural Science Foundation of China[U1432248] ; National Key Projects of Research and Development[2016YFC0904600] ; National Natural Science Foundation of China[11305226] ; National Natural Science Foundation of China[11605255] ; Western Talent Program of Chinese Academy of Sciences[2013-165] ; Western Talent Program of Chinese Academy of Sciences[Y460030XB0] ; Scientific Technology Research Projects of Lanzhou City[2015-3-75] ; Scientific Technology Research Projects of Lanzhou City[Y660040SJ0]
WOS关键词	MONOXIDE-RELEASING MOLECULES ; ENDOTHELIAL-CELL APOPTOSIS ; DNA-DAMAGE ; IN-VIVO ; OXIDATIVE STRESS ; THERAPEUTIC APPLICATIONS ; CYTOCHROME-C ; PHYSIOLOGICAL FUNCTIONS ; ULTRAVIOLET-RADIATION ; PARTICLE IRRADIATION
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000390739600005
资助机构	Key Program of National Natural Science Foundation of China ; National Key Projects of Research and Development ; National Natural Science Foundation of China ; Western Talent Program of Chinese Academy of Sciences ; Scientific Technology Research Projects of Lanzhou City
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/43993]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 5.Lanzhou Univ, Sch Hosp Stomatol, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Rong,Song, Jing'e,Si, Jing,et al. Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2016,793:41-50.
APA	Zhou, Rong.,Song, Jing'e.,Si, Jing.,Zhang, Hong.,Liu, Bin.,...&Zhang, Qianjing.(2016).Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,793,41-50.
MLA	Zhou, Rong,et al."Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 793(2016):41-50.