SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato

doi:10.1016/j.plantsci.2016.09.005

	SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato
	Ren, Zhenxin1 ; Wang, Xiaomin 2
刊名	PLANT SCIENCE
	2016-12-01
卷号	253 页码:13-20
关键词	SlTIR1 Auxin signaling Hormone crosstalk Root formation Stenospermocarpic fruit
ISSN号	0168-9452
DOI	10.1016/j.plantsci.2016.09.005
英文摘要	TIR1 and its homologs act as auxin receptors and play important roles in plant growth and development in Arabidopsis thaliana. An auxin receptor homolog Solanum lycopersicum TIR1 (SlTIR1) has been isolated from tomato cultivar Micro-Tom, and SlTIR1 over-expression results in parthenocarpic fruit formation. In this study, the promoter driving the beta-glucuronidase (GUS) expression vector was constructed and stably transformed into Micro-Tom seedlings. The SlTIR1 promoter driving GUS expression accumulated predominantly in the leaf and vasculature in transgenic seedlings. Promoter analysis identified an auxin-response element (AuxRE) and two gibberellic acid (GA)-response elements in the SlTIR1 promoter. Quantitative PCR showed that SlTIR1 transcript level was down-regulated by naphthaleneacetic acid, ethephon and abscisic acid and up-regulated by GA. Furthermore, because of the lack of ability to form reproductive seeds in SlTIR1 over-expressing Micro-Tom, this limits further exploration of potential roles of SlTIR1 in auxin signaling. Here, an antisense vector and an over-expression vector of the SlTIR1 gene were stably transformed into Micro-Tom and Ailsa Craig tomato, respectively. Phenotypes and physiological analyses indicated that SlTIR1 regulated primary root growth and auxin-associated lateral root formation in Micro-Tom. Meanwhile, SlTIR1 also stimulated abnormal seed development, so-called stenospermocarpy, in Ailsa Craig. Transcript accumulations of auxin-signaling genes determined by quantitative PCR were consistent with the idea that SlTIR1 regulated plant growth and development, partially mediated by controlling the mRNA levels of auxin-signaling genes. Our work demonstrates that SlTIR1 regulated auxin-induced root growth and stimulated stenospermocarpic fruit formation. SlTIR1 may be a key mediator of the crosstalk among auxin and other hormones to co-regulate plant growth and development. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[31360485] ; Guangxi Natural Science Foundation[2012GXNSFBA053076]
WOS关键词	BOX PROTEIN TIR1 ; LEAF MORPHOGENESIS ; PEA FRUIT ; GIBBERELLIN ; ARABIDOPSIS ; RECEPTOR ; EXPRESSION ; FAMILY ; SET ; DEGRADATION
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000388049000002
资助机构	National Natural Science Foundation of China ; Guangxi Natural Science Foundation
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/43696]
专题	中国科学院近代物理研究所
通讯作者	Ren, Zhenxin; Wang, Xiaomin
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Space Radiobiol, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 2.Yulin Normal Univ, Coll Biol & Pharm, Yulin 537000, Peoples R China
推荐引用方式 GB/T 7714	Ren, Zhenxin,Wang, Xiaomin. SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato[J]. PLANT SCIENCE,2016,253:13-20.
APA	Ren, Zhenxin,&Wang, Xiaomin.(2016).SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato.PLANT SCIENCE,253,13-20.
MLA	Ren, Zhenxin,et al."SlTIR1 is involved in crosstalk of phytohormones, regulates auxin-induced root growth and stimulates stenospermocarpic fruit formation in tomato".PLANT SCIENCE 253(2016):13-20.