Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation

doi:10.1371/journal.pone.0155725

	Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation
	Zhang, Xurui 1,2,3; Ye, Caiyong 1,2; Sun, Fang 1,2,3; Wei, Wenjun 1,2,3; Hu, Burong 1,2; Wang, Jufang 1,2
刊名	PLOS ONE
	2016-05-17
卷号	11 页码:16
ISSN号	1932-6203
DOI	10.1371/journal.pone.0155725
英文摘要	Persistent DNA damage is considered as a main cause of cellular senescence induced by ionizing radiation. However, the molecular bases of the DNA damage and their contribution to cellular senescence are not completely clear. In this study, we found that both heavy ions and X-rays induced senescence in human uveal melanoma 92-1 cells. By measuring senescence associated-beta-galactosidase and cell proliferation, we identified that heavy ions were more effective at inducing senescence than X-rays. We observed less efficient repair when DNA damage was induced by heavy ions compared with X-rays and most of the irreparable damage was complex of single strand breaks and double strand breaks, while DNA damage induced by X-rays was mostly repaired in 24 hours and the remained damage was preferentially associated with telomeric DNA. Our results suggest that DNA damage induced by heavy ion is often complex and difficult to repair, thus presents as persistent DNA damage and pushes the cell into senescence. In contrast, persistent DNA damage induced by X-rays is preferentially associated with telomeric DNA and the telomere-favored persistent DNA damage contributes to X-rays induced cellular senescence. These findings provide new insight into the understanding of high relative biological effectiveness of heavy ions relevant to cancer therapy and space radiation research.
资助项目	National Natural Science Foundation of China[U1232125] ; National Natural Science Foundation of China[31270895] ; National Natural Science Foundation of China[U1432121]
WOS关键词	DOUBLE-STRAND BREAKS ; CELLS ; REPAIR ; HETEROCHROMATIN ; FIBROBLASTS ; MELANOMA ; PATHWAY ; ARREST ; CANCER ; ATM
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000376282300063
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/42671]
专题	中国科学院近代物理研究所
通讯作者	Wang, Jufang
作者单位	1.Chinese Acad Sci, Gansu Key Lab Space Radiobiol, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xurui,Ye, Caiyong,Sun, Fang,et al. Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation[J]. PLOS ONE,2016,11:16.
APA	Zhang, Xurui,Ye, Caiyong,Sun, Fang,Wei, Wenjun,Hu, Burong,&Wang, Jufang.(2016).Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation.PLOS ONE,11,16.
MLA	Zhang, Xurui,et al."Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation".PLOS ONE 11(2016):16.