Apoptosis of murine melanoma cells induced by heavy-ion radiation combined with Tp53 gene transfer

doi:10.1080/09553000801902117

	Apoptosis of murine melanoma cells induced by heavy-ion radiation combined with Tp53 gene transfer
	Duan, Xin 1,2; Zhang, Hong1 ; Liu, Bing 1; Li, Xiao-Da 1; Gao, Qing-Xiang 3; Wu, Zheng-Hua 1
刊名	INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
	2008-03-01
卷号	84 页码:211-217
关键词	Tp53 carbon-ion beam apoptosis melanoma
ISSN号	0955-3002
DOI	10.1080/09553000801902117
英文摘要	Purpose: To investigate the effect of exogenous wild type p53 (Tp53) on murine melanoma B 16 cell apoptosis induced by carbon-ion beam (C-beam) irradiation. Materials and methods: The murine cell line B 16, which has wild-type Tp53 gene status was studied, as well as B 16 cells transfected with an adenoviral vector containing the wild-type Tp53 gene (B16/Tp53). Cells were irradiated with C-beam and assayed for cell survival (colony-forming assay), cellular morphology (acridine orange assay), the frequency of apoptotic cells (fluorescence microscopy) and protein expression (Western blot analysis). Results: The radiosensitivity of B 16/Tp53 cells was significantly higher than that of B 16 cells. In contrast with Tp53 transfer alone, the combination of C-bearn with Tp53 transfer induced a higher proportion of apoptotic cells and micronuclei. After C-beam irradiation, there was no significant increased expression of the cyclin-dependent kinase inhibitor p21 and Tp53 in B16/Tp53 cells compared to B16 cells, but a decreased expression of murine double minute-2 (Mdm2) was observed. Conclusion: The results of this study suggest the potential application of C-beam combined with Tp53 in the treatment of melanoma in human patients.
WOS关键词	WILD-TYPE P53 ; CANCER CELLS ; IONIZING-RADIATION ; DNA-REPAIR ; THERAPY ; RADIOTHERAPY ; EXPRESSION ; CARCINOMA ; GROWTH ; P21(WAF1/CIP1)
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Nuclear Science & Technology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000254190700004
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/28580]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing, Peoples R China 3.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Duan, Xin,Zhang, Hong,Liu, Bing,et al. Apoptosis of murine melanoma cells induced by heavy-ion radiation combined with Tp53 gene transfer[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,2008,84:211-217.
APA	Duan, Xin,Zhang, Hong,Liu, Bing,Li, Xiao-Da,Gao, Qing-Xiang,&Wu, Zheng-Hua.(2008).Apoptosis of murine melanoma cells induced by heavy-ion radiation combined with Tp53 gene transfer.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,84,211-217.
MLA	Duan, Xin,et al."Apoptosis of murine melanoma cells induced by heavy-ion radiation combined with Tp53 gene transfer".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 84(2008):211-217.