Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae | |
Guo Minrui1; Chen Guogang1; Chen Jiluan1; Zheng Minggang2 | |
刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA |
2019-10 | |
卷号 | 18期号:5页码:1199-1206 |
关键词 | Nannochloropsis sp long-chain fatty acid elongase Saccharomyces cerevisiae fatty acid production |
ISSN号 | 1672-5182 |
DOI | 10.1007/s11802-019-3946-y |
英文摘要 | The marine microalga Nannochloropsis sp. contains various elongases and desaturases that are critical for biosynthesis of polyunsaturated fatty acids. A full-length cDNA encoding a long-chain fatty acid elongase, named NsFAE, was cloned from Nannochloropsis sp.. The open reading frame of NsFAE (GenBank accession no. MF680548) consisted of 1068bp and encoded a predicted protein of 355 amino acids with molecular mass 38.8kDa. The deduced polypeptide showed 43%-44% identity to fatty acyl elongases from other algae. RT-PCR experiments indicated that the NsFAE gene exhibited the highest expression in Nannochloropsis sp. at 72h (i.e., during the third growth stage) and the expression was significantly lower in the other four growth stages. Plasmid pNsFAE-CRISPR and a recombinant DNA fragment (ADH(1)p-NsFAE-CYCt) were transformed into Saccharomyces cerevisiae strain BY4742 using the CRISPR-Cas system. Yeast transformants containing NsFAE produced three fatty acids not normally present in wild-type BY4742-linoleic acid, linolenic acid and eicosadienoic acid-indicating that NsFAE encodes a functional elongase enzyme. |
资助项目 | Basic Scientific Fund for National Public Research Institutes of China[2016Q07] |
WOS研究方向 | Oceanography |
语种 | 英语 |
出版者 | OCEAN UNIV CHINA |
WOS记录号 | WOS:000482849800024 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/30110] |
专题 | 业务部门_海洋生态研究中心 |
通讯作者 | Chen Guogang |
作者单位 | 1.Shihezi Univ, Coll Food, Shihezi 832000, Peoples R China 2.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Guo Minrui,Chen Guogang,Chen Jiluan,et al. Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2019,18(5):1199-1206. |
APA | Guo Minrui,Chen Guogang,Chen Jiluan,&Zheng Minggang.(2019).Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae.JOURNAL OF OCEAN UNIVERSITY OF CHINA,18(5),1199-1206. |
MLA | Guo Minrui,et al."Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae".JOURNAL OF OCEAN UNIVERSITY OF CHINA 18.5(2019):1199-1206. |
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