Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5

doi:10.1007/s13131-018-1226-0

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5
	Chao, Yaxi 1; Wang, Shuyan 1; Wu, Suqi 1,2; Wei, Jiaqiang 1; Chen, Hao 1,2
刊名	ACTA OCEANOLOGICA SINICA
	2019-06
卷号	38 期号:6 页码:65-74
关键词	alginate lyase Vibrio sp alginate poly--L-guluronic acid lyase oligosaccharides
ISSN号	0253-505X
DOI	10.1007/s13131-018-1226-0
英文摘要	Seven bacterial clones with alginate-utilizing activity were isolated from rotten kelp. By activity test, the Vibrio sp. QD-5 with the potential alginate-degrading capability was chosen to carry out the draft genome sequencing, and the result showed that the Vibrio sp. QD-5 containing an alginate lyase gene cluster. One of these genes, aly-IV, was cloned and characterized for the first time. After overexpression, Aly-IV, with a molecular mass of about 62 kDa and a theoretical isoelectric point (pI) of 5.12, was purified to a specific activity of 1 256.78 U/mg and showed highest activity at 35 degrees C in the Tris-HCl buffer at pH of 8.9. Moreover, the enzyme activity was enhanced by the metal ions of Na+, K+ and Mg2+ under certain concentration. Aly-IV degraded favorably polyG blocks in an endo-type, yielding monomer and dimer as the main products. Due to its high substrate specificity, Aly-IV could be used as a potential tool for production of polyG oligosaccharides with low degree of polymerization (DP) and for determining the fine structure of alginate.
资助项目	Scientific and Technological Innovation Project financially of Qingdao National Laboratory for Marine Science and Technology[2016ASKJ14]
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000470817800007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/21538]
专题	业务部门_海洋生态研究中心
通讯作者	Chen, Hao
作者单位	1.Minist Nat Resources, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chao, Yaxi,Wang, Shuyan,Wu, Suqi,et al. Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5[J]. ACTA OCEANOLOGICA SINICA,2019,38(6):65-74.
APA	Chao, Yaxi,Wang, Shuyan,Wu, Suqi,Wei, Jiaqiang,&Chen, Hao.(2019).Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5.ACTA OCEANOLOGICA SINICA,38(6),65-74.
MLA	Chao, Yaxi,et al."Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5".ACTA OCEANOLOGICA SINICA 38.6(2019):65-74.