The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis

doi:10.1007/s00343-019-7304-0

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	The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis
	Li Wanjuan 1; Xue Shenglun 1; Pang Min 2; Yue Zonghao 1; Yang Dazuo 1; Zhou Yibing 1; Zhao Huan 1
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2018-11
卷号	36 期号:6 页码:2297-2307
关键词	Perinereis aibuhitensis vitellogenin B(a)p expression profiles
ISSN号	2096-5508
DOI	10.1007/s00343-019-7304-0
英文摘要	In order to investigate the endocrine toxicity of B(a)p to marine polychaete Perinereis aibuhitensis, vitellogenin (VTG) cDNA from the P. aibuhitensis was isolated, recombinated and expressed for the first time. The full length P. aibuhitensis vitellogenin gene (PaVTG) was 5 325 bp, and encoded 1 692 amino acids. It contained the vitellogenin_N domain of unknown function (DUF1943), a von Willebrand factor type D domain, as well as a conserved KALGNAG motif. The expression of VTG gene and protein were mainly up-regulated after exposed to B(a)p at transcriptional and translational levels. PaVTG gene expression did not change significantly at day 4. At day 7 PaVTG expression was up-regulated in 0.5 mu g/L and 5 mu g/L B(a)p group. At day 14 PaVTG was significantly up-regulated in 0.5-10 mu g/L B(a)p. The protein expression of PaVTG in 0.5 mu g/L and 10 mu g/L B(a)p group was up-regulated with time prolonging, but the expression in 5 mu g/L and 50 mu g/L B(a)p group exhibited first increased and then decreased trend. With the increasing of B(a)p concentration PaVTG mRNA and protein expression both firstly increased then decreased. In contrast to B(a)p exposure, estradiol did not induce PaVTG gene and protein expression, until late times of exposure (14 d). Overall, the results in this study indicate that PaVTG could be used as a potential indicator of the effects environmental estrogenic compounds.
资助项目	Foundation of Education Department of Liaoning Province[L201609]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000454629000032
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/10904]
专题	业务部门_海洋生态研究中心
通讯作者	Zhao Huan
作者单位	1.Dalian Ocean Univ, Key Lab Marine Bioresource Restorat & Habitat Rep, Dalian 116023, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Ecol & Environm Sci & Engn, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Li Wanjuan,Xue Shenglun,Pang Min,et al. The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6):2297-2307.
APA	Li Wanjuan.,Xue Shenglun.,Pang Min.,Yue Zonghao.,Yang Dazuo.,...&Zhao Huan.(2018).The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6),2297-2307.
MLA	Li Wanjuan,et al."The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6(2018):2297-2307.