A case study on chemical defense based on quorum sensing: antibacterial activity of sponge-associated bacterium Pseudoalteromonas sp NJ6-3-1 induced by quorum sensing mechanisms

doi:10.1007/s13213-010-0129-x

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	A case study on chemical defense based on quorum sensing: antibacterial activity of sponge-associated bacterium Pseudoalteromonas sp NJ6-3-1 induced by quorum sensing mechanisms
	Guo, Xiuchun 1,2; Zheng, Li 1; Zhou, Wenhui 1,2; Cui, Zhisong 1; Han, Ping 1; Tian, Li 1; Wang, Xiaoru 1,2
刊名	ANNALS OF MICROBIOLOGY
	2011-06
卷号	61 期号:2 页码:247-255
关键词	Quorum sensing Sponge-associated bacterium Antibacterial activity Diketoperazines
ISSN号	1590-4261
DOI	10.1007/s13213-010-0129-x
英文摘要	A case study to investigate the relationship between antibacterial activity and quorum sensing mechanisms was carried out on a sponge-associated bacterium with remarkable biological activities: Pseudoalteromonas sp. NJ6-3-1. The dependence of active substance production on cell density was studied under various growth conditions. Bacteria NJ6-3-1 was found to start producing antibacterial compounds only when cell density reached the threshold value of OD(630) = 0.4. To simulate the competitive real marine environment, NJ6-3-1 at low cell density (OD(630) value below the required threshold value) was co-cultured with the terrestrial bacterium Staphylococcus aureus. Antibacterial activity assays indicated the existence of some signal molecules in the metabolites of S. aureus that could induce NJ6-3-1 to produce antibacterial substances even at low cell density. Three diketopiperazines (DKPs) as metabolites and potential autoinducers of NJ6-3-1 were synthesized and co-cultured with low density NJ6-3-1. The antibacterial activity assay showed that one of these DKPs-cyclo-(l-Phe-l-Val)-was the autoinducer and could indeed induce NJ6-3-1 to produce antibacterial substances under low cell density. Our results thus provide preliminary support to the hypothesis that the antibacterial activity of NJ6-3-1 is controlled by the quorum sensing system in both an intra-species and an inter-species manner.
资助项目	Qingdao government[08-1-3-10-JCH]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000290319900005
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4217]
专题	业务部门_海洋生态研究中心
作者单位	1.SOA, Inst Oceanog 1, Marine Ecol Ctr, Qingdao 266061, Peoples R China; 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Guo, Xiuchun,Zheng, Li,Zhou, Wenhui,et al. A case study on chemical defense based on quorum sensing: antibacterial activity of sponge-associated bacterium Pseudoalteromonas sp NJ6-3-1 induced by quorum sensing mechanisms[J]. ANNALS OF MICROBIOLOGY,2011,61(2):247-255.
APA	Guo, Xiuchun.,Zheng, Li.,Zhou, Wenhui.,Cui, Zhisong.,Han, Ping.,...&Wang, Xiaoru.(2011).A case study on chemical defense based on quorum sensing: antibacterial activity of sponge-associated bacterium Pseudoalteromonas sp NJ6-3-1 induced by quorum sensing mechanisms.ANNALS OF MICROBIOLOGY,61(2),247-255.
MLA	Guo, Xiuchun,et al."A case study on chemical defense based on quorum sensing: antibacterial activity of sponge-associated bacterium Pseudoalteromonas sp NJ6-3-1 induced by quorum sensing mechanisms".ANNALS OF MICROBIOLOGY 61.2(2011):247-255.