Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation

doi:10.1007/s00792-012-0441-4

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation
	Liu, Chenlin; Wu, Guangting; Huang, Xiaohang; Liu, Shenghao; Cong, Bailin
刊名	EXTREMOPHILES
	2012-05
卷号	16 期号:3 页码:419-425
关键词	Chlamydomonas sp ICE-L Freezing acclimation Reference genes Real-time RT-PCR Normalization FAD3
ISSN号	1431-0651
DOI	10.1007/s00792-012-0441-4
英文摘要	Antarctic ice alga sp. ICE-L can endure extreme low temperature and high salinity stress under freezing conditions. To elucidate the molecular acclimation mechanisms using gene expression analysis, the expression stabilities of ten housekeeping genes of sp. ICE-L during freezing stress were analyzed. Some discrepancies were detected in the ranking of the candidate reference genes between geNorm and NormFinder programs, but there was substantial agreement between the groups of genes with the most and the least stable expression. RPL19 was ranked as the best candidate reference genes. Pairwise variation () analysis indicated the combination of two reference genes was sufficient for qRT-PCR data normalization under the experimental conditions. Considering the co-regulation between RPL19 and RPL32 (the most stable gene pairs given by geNorm program), we propose that the mean data rendered by RPL19 and GAPDH (the most stable gene pairs given by NormFinder program) be used to normalize gene expression values in sp. ICE-L more accurately. The example of FAD3 gene expression calculation demonstrated the importance of selecting an appropriate category and number of reference genes to achieve an accurate and reliable normalization of gene expression during freeze acclimation in sp. ICE-L.
资助项目	National Natural Science Foundation[40876106]
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
语种	英语
出版者	SPRINGER TOKYO
WOS记录号	WOS:000303870300007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4116]
专题	业务部门_海洋生态研究中心
作者单位	State Ocean Adm, Inst Oceanog 1, Marine Ecol Ctr, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Liu, Chenlin,Wu, Guangting,Huang, Xiaohang,et al. Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation[J]. EXTREMOPHILES,2012,16(3):419-425.
APA	Liu, Chenlin,Wu, Guangting,Huang, Xiaohang,Liu, Shenghao,&Cong, Bailin.(2012).Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation.EXTREMOPHILES,16(3),419-425.
MLA	Liu, Chenlin,et al."Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation".EXTREMOPHILES 16.3(2012):419-425.