Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods

doi:10.1271/bbb.120104

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	Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods
	Wang, Quanfu 1; Zhang, Chunyu 1; Hou, Yanhua 1; Lin, Xuezheng 2; Shen, Jihong 2; Guan, Xiangyu 3
刊名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
	2013-01
卷号	77 期号:1 页码:17-21
关键词	cold-active lipase psychrophilic bacterium optimization Plackett-Burman design response surface methodology
ISSN号	0916-8451
DOI	10.1271/bbb.120104
英文摘要	Statistical experimental designs were applied to optimize cold-active lipase production by the psychrophilic bacterium Moritella sp. 2-5-10-1. First, a Plackett-Burmen design (PBD) was used to evaluate the significant effects of various fermentation parameters. The results indicated that soybean meal, temperature, and Tween-80 had significant influences on lipase production. The levels of these variables were optimized subsequently using central composite design (CCD). A quadratic regression model of cold-active lipase production was built, and verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using optimized conditions, cold-active lipase production (30.56 U/mL) was obtained. The results clearly indicated that the model was adequate even on a large scale. To our knowledge, this is the first report of statistical optimization of cold-active lipase production by a psychrophilic bacterium.
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资助项目	Natural Scientific Research Innovation Foundation at the Harbin Institute of Technology[HIT-NSRIF201011]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000316164800003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4020]
专题	业务部门_海洋生态研究中心
作者单位	1.Harbin Inst Technol, Sch Marine & Technol, Weihai 264209, Peoples R China; 2.First Inst Oceanog, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China; 3.China Univ Geosci, Sch Ocean Sci, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Wang, Quanfu,Zhang, Chunyu,Hou, Yanhua,et al. Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2013,77(1):17-21.
APA	Wang, Quanfu,Zhang, Chunyu,Hou, Yanhua,Lin, Xuezheng,Shen, Jihong,&Guan, Xiangyu.(2013).Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,77(1),17-21.
MLA	Wang, Quanfu,et al."Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77.1(2013):17-21.