Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation

doi:10.1007/s10529-013-1386-0

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	Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation
	Huang, Yi-jiang 1; Wang, Ling 2; Zheng, Ming-gang 3; Zheng, Li 3; Tong, Yan-li 3; Li, Yan 2
刊名	BIOTECHNOLOGY LETTERS
	2014-03
卷号	36 期号:3 页码:575-579
关键词	Acetyl-CoA carboxylase Acyl-CoA:diacylglycerol acyl-transferase Aureochrome-1 Lipid Long-chain-acyl CoA synthetase Nannochloropsis gaditana Transcription factor
ISSN号	0141-5492
DOI	10.1007/s10529-013-1386-0
英文摘要	Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and report that the lipid content in recombinant Saccharomyces cerevisiae was 1.6-fold more than in wild-type S. cerevisiae (6.3 % lipid increased to 10 %). Over-expression of AUREO1 in S. cerevisiae up-regulated the expression of acetyl-CoA carboxylase and acyl-CoA:diacylglycerol acyl-transferase but down-regulated the expression of long-chain-acyl CoA synthetase. This enhanced the accumulation of lipid. This study highlights a novel function of AUREO1 and allows a better understanding of the regulation mechanism of fatty acid metabolism.
电子版国际标准刊号	15736776
资助项目	Marine Renewable Energy Funds Project[201305022] ; Marine Renewable Energy Funds Project[GHME2001SW02]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000333349300019
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3854]
专题	业务部门_海洋生态研究中心
作者单位	1.Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; 2.Qingdao Univ, Coll Chem Engn, Qingdao 266071, Peoples R China; 3.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yi-jiang,Wang, Ling,Zheng, Ming-gang,et al. Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation[J]. BIOTECHNOLOGY LETTERS,2014,36(3):575-579.
APA	Huang, Yi-jiang,Wang, Ling,Zheng, Ming-gang,Zheng, Li,Tong, Yan-li,&Li, Yan.(2014).Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation.BIOTECHNOLOGY LETTERS,36(3),575-579.
MLA	Huang, Yi-jiang,et al."Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation".BIOTECHNOLOGY LETTERS 36.3(2014):575-579.