Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress

doi:10.1016/j.gene.2014.03.026

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress
	Liu, Shenghao 1,2; Ju, Jianfang 1; Xia, Guangmin 1
刊名	GENE
	2014-06-10
卷号	543 期号:1 页码:145-152
关键词	Abiotic stress Antarctic moss Real-time PCR Flavonoid hydroxylase Phylogenetic analysis
ISSN号	0378-1119
DOI	10.1016/j.gene.2014.03.026
英文摘要	Flavonoids are ubiquitous plant secondary metabolites, and their hydroxylation pattern determines their color, stability, and antioxidant capacity. The hydroxylation pattern of the B-ring of flavonoids is determined by the activity of two members of cytochrome P450 protein (P450) family, the flavonoid 3 '-hydroxylase (F3 ' H) and flavonoid 3 ',5 '-hydroxylase (F3 ',5 ' H). However, they are still not well documented in lower plants such as bryophytes. We report the identification of gene encoding F3 ' H, F3',5 ' H from Antarctic moss Pohlia nutans and their transcriptional regulation under different stress conditions. Totally, sixteen cDNAs were isolated from P. nutans by RT-PCR and RACE techniques, all of which were predicted to code for F3 ' Hs or F3 ',5 ' Hs based on their annotations of Blast results. Amino acid alignment showed that they possessed the featured conserved domains of flavonoid hydroxylase, including proline-rich "hinge" region, EXXR motif, oxygen binding pocket motif, heme binding domain and substrate recognition sites. Phylogenetic analysis indicated that moss F3 ' Hs and F3 ',5 ' Hs were highly conserved and have independent evolution from the monocots, dicots and ferns. Meanwhile, real-time PCR analysis revealed that the expreSsion profiling of flavonoid hydroxylase genes was influenced by diverse abiotic stresses including cold, salinity, drought or UV-B radiation and plant hormone abscisic acid (ABA) or jasmonic acid apo treatment. Since 3 ',4 ',5 '-hydroxylated flavonoid-derivatives may serve a multitude of functions, including antioxidant activity and UV filters, the evolution and expression profile of flavonoid hydroxylase probably reflect the adaptive value of Antarctic moss in the acclimation of polar environment. (C)2014 Elsevier B.V. All rights reserved.
资助项目	National Transgenic Project[2013ZX08002002-003]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000336355500020
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3821]
专题	业务部门_海洋生态研究中心
作者单位	1.Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Peoples R China; 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Substance, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shenghao,Ju, Jianfang,Xia, Guangmin. Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress[J]. GENE,2014,543(1):145-152.
APA	Liu, Shenghao,Ju, Jianfang,&Xia, Guangmin.(2014).Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress.GENE,543(1),145-152.
MLA	Liu, Shenghao,et al."Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress".GENE 543.1(2014):145-152.