Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply

doi:10.1007/s00299-015-1892-8

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	Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply
	Liu, Xiang 1; Wu, Fei-Hua 1,2; Li, Jing-Xi 3; Chen, Juan 1; Wang, Guang-Hui 1; Wang, Wen-Hua 5; Hu, Wen-Jun 1,4; Gao, Li-Jie 3; Wang, Zong-Ling 3; Chen, Jun-Hui 3
刊名	PLANT CELL REPORTS
	2016-02
卷号	35 期号:2 页码:397-413
关键词	Antioxidative defense Arabidopsis Cadmium toxicity Glutathione homeostasis Sulfate transporter Sulfur assimilation-related genes
ISSN号	0721-7714
DOI	10.1007/s00299-015-1892-8
英文摘要	In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synthesized by sulfur (S) assimilation pathway. Many studies have tried to investigate the role of GSH homeostasis on Cd tolerance using mutants; however, most of them have focused on the last few steps of S assimilation. Until now, mutant evidence that explored the relationship between GSH homeostasis on Cd tolerance and S absorption is rare. To further reveal the role of GSH homeostasis on Cd stress, the wild-type and a sultr1;1-sultr1;2 double mutant which had a defect in two distinct high-affinity sulfate transporters were used in this study. Growth parameters, biochemical or zymological indexes and S assimilation-related genes' expression were compared between the mutant and wild-type Arabidopsis plants. It was found that the mutations of SULTR1;1 and SULTR1;2 did not affect Cd accumulation. Compared to the wild-type, the double mutant was more sensitive to Cd under limited sulfate supply and suffered from stronger oxidative damage. More importantly, under the same condition, lower capacity of S assimilation resulted in decreased GSH content in mutant. Faced to the limited GSH accumulation, mutant seedlings consumed a large majority of GSH in pool for the synthesis of phytochelatins rather than participating in the antioxidative defense. Therefore, homeostasis of GSH, imbalance between antioxidative defense and severe oxidative damage led to hypersensitivity of double mutant to Cd under limited sulfate supply.
资助项目	China Postdoctoral Science Foundation[2012M521278]
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000371240400010
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3514]
专题	业务部门_海洋生态研究中心
作者单位	1.Xiamen Univ, Coll Environm & Ecol, Key Lab Subtrop Wetland Ecosyst Res MOE, Xiamen 361102, Fujian, Peoples R China; 2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China; 3.State Ocean Adm, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Shandong, Peoples R China; 4.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China; 5.Fujian Inst Subtrop Bot, Fujian Key Lab Subtrop Plant Physiol & Biochem, Xiamen 361005, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiang,Wu, Fei-Hua,Li, Jing-Xi,et al. Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply[J]. PLANT CELL REPORTS,2016,35(2):397-413.
APA	Liu, Xiang.,Wu, Fei-Hua.,Li, Jing-Xi.,Chen, Juan.,Wang, Guang-Hui.,...&Zheng, Hai-Lei.(2016).Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply.PLANT CELL REPORTS,35(2),397-413.
MLA	Liu, Xiang,et al."Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply".PLANT CELL REPORTS 35.2(2016):397-413.