Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp B-3

doi:10.1007/s00343-016-4393-x

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp B-3
	Yu Leiye 1; Sun Guojie 1; Wei Jingfang 1; Wang Yingze 1; Du Chao 1; Li Jiang 2
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2016-09
卷号	34 期号:5 页码:1064-1071
关键词	exopolysaccharide (EPS) Antarctic bacterium RAW264.7 TLR4 NF-kappa B
ISSN号	0254-4059
DOI	10.1007/s00343-016-4393-x
英文摘要	An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF) a secretion, and phagocytic ability. Furthermore, TAK-242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and I kappa B alpha degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-kappa B signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response.
资助项目	National Natural Science Foundation of China[31100715]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000379737000018
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3360]
专题	业务部门_海洋生态研究中心
作者单位	1.Hebei Univ Sci & Technol, Coll Bioscience & Bioengn, Shijiazhuang 050018, Peoples R China; 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Yu Leiye,Sun Guojie,Wei Jingfang,et al. Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp B-3[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1064-1071.
APA	Yu Leiye,Sun Guojie,Wei Jingfang,Wang Yingze,Du Chao,&Li Jiang.(2016).Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp B-3.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1064-1071.
MLA	Yu Leiye,et al."Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp B-3".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1064-1071.