Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae

doi:10.1007/s11802-019-3946-y

	Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae
	Guo Minrui 1; Chen Guogang 1; Chen Jiluan 1; Zheng Minggang 2
刊名	JOURNAL OF OCEAN UNIVERSITY OF CHINA
	2019-10
卷号	18 期号:5 页码:1199-1206
关键词	Nannochloropsis sp long-chain fatty acid elongase Saccharomyces cerevisiae fatty acid production
ISSN号	1672-5182
DOI	10.1007/s11802-019-3946-y
英文摘要	The marine microalga Nannochloropsis sp. contains various elongases and desaturases that are critical for biosynthesis of polyunsaturated fatty acids. A full-length cDNA encoding a long-chain fatty acid elongase, named NsFAE, was cloned from Nannochloropsis sp.. The open reading frame of NsFAE (GenBank accession no. MF680548) consisted of 1068bp and encoded a predicted protein of 355 amino acids with molecular mass 38.8kDa. The deduced polypeptide showed 43%-44% identity to fatty acyl elongases from other algae. RT-PCR experiments indicated that the NsFAE gene exhibited the highest expression in Nannochloropsis sp. at 72h (i.e., during the third growth stage) and the expression was significantly lower in the other four growth stages. Plasmid pNsFAE-CRISPR and a recombinant DNA fragment (ADH(1)p-NsFAE-CYCt) were transformed into Saccharomyces cerevisiae strain BY4742 using the CRISPR-Cas system. Yeast transformants containing NsFAE produced three fatty acids not normally present in wild-type BY4742-linoleic acid, linolenic acid and eicosadienoic acid-indicating that NsFAE encodes a functional elongase enzyme.
资助项目	Basic Scientific Fund for National Public Research Institutes of China[2016Q07]
WOS关键词	FUNCTIONAL-CHARACTERIZATION ; C20-ELONGASE GENE ; MARINE MICROALGAE ; PAVLOVA-VIRIDIS ; DESATURASE GENE ; DHA
WOS研究方向	Oceanography
语种	英语
出版者	OCEAN UNIV CHINA
WOS记录号	WOS:000482849800024
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/30070]
专题	自然资源部第一海洋研究所
通讯作者	Chen Guogang
作者单位	1.Shihezi Univ, Coll Food, Shihezi 832000, Peoples R China 2.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Guo Minrui,Chen Guogang,Chen Jiluan,et al. Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2019,18(5):1199-1206.
APA	Guo Minrui,Chen Guogang,Chen Jiluan,&Zheng Minggang.(2019).Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae.JOURNAL OF OCEAN UNIVERSITY OF CHINA,18(5),1199-1206.
MLA	Guo Minrui,et al."Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae".JOURNAL OF OCEAN UNIVERSITY OF CHINA 18.5(2019):1199-1206.