Molecular cloning and expression analysis of major intrinsic protein gene in Chlamydomonas sp ICE-L from Antarctica

doi:10.1007/s00792-017-0945-z

	Molecular cloning and expression analysis of major intrinsic protein gene in Chlamydomonas sp ICE-L from Antarctica
	Li, Lulu 1; An, Meiling 2; Qu, Changfeng 1; Zheng, Zhou 1; Wang, Yibin 1; Liu, Fangming 1; He, Yingying 1; He, Xiaodong 1; Miao, Jinlai 1,2,3
刊名	EXTREMOPHILES
	2017-07
卷号	21 期号:4 页码:817-827
关键词	Chlamydomonas sp. ICE-L CiMIP1 gene qRT-PCR Expression analysis NPA motif Ar/R selectivity filter
ISSN号	1431-0651
DOI	10.1007/s00792-017-0945-z
英文摘要	Major intrinsic proteins (MIPs) form channels facilitating the passive transport of water and other small polar molecules across membranes. In this study, the complete open reading frame (ORF) of CiMIP1 (GenBank ID KY316061) encoding one kind of MIPs in the Antarctic ice microalga Chlamydomonas sp. ICE-L is successfully cloned using RACE. In addition, the expression patterns of CiMIP1 gene under different conditions of temperature and salinity are determined by qRT-PCR. The ORF of CiMIP1 gene encodes 308 amino acids, and the deduced amino acid sequence shows 74% homology with Chlamydomonas reinhardtii CrMIP1 (GenBank number 159471952). Phylogenetic analysis reveals that algal MIPs are divided into seven groups, and it is speculated that CiMIP1 most likely belongs to the MIPD subfamily. In addition, we are surprised to find that a third NPA motif exists at the carboxy terminus of the target protein except for two highly conserved ones. Expression analysis shows that the transcriptional levels of CiMIP1 gene are upregulated under either lower temperature or higher temperature and high salinity. In summary, the results together have provide new insights into the newly discovered gene in green algae and lay the foundation for further studies on the adaptation mechanism of Chlamydomonas sp. ICE-L to abiotic stresses.
资助项目	Qingdao Entrepreneurship and Innovation Pioneers Program[15-10-3-15-(44)-zch]
WOS关键词	AROMATIC/ARGININE SELECTIVITY FILTER ; WATER CHANNEL ; AQUAPORIN FAMILY ; MEMBRANE ; TRANSPORT ; RICE ; ARABIDOPSIS ; EVOLUTION ; IDENTIFICATION ; GLYCEROL
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
语种	英语
出版者	SPRINGER JAPAN KK
WOS记录号	WOS:000403945900013
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27497]
专题	自然资源部第一海洋研究所
通讯作者	Miao, Jinlai
作者单位	1.First Inst Oceanog, State Ocean Adm, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.Qingdao Univ, Coll Med, Ningxia Rd 308th, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Li, Lulu,An, Meiling,Qu, Changfeng,et al. Molecular cloning and expression analysis of major intrinsic protein gene in Chlamydomonas sp ICE-L from Antarctica[J]. EXTREMOPHILES,2017,21(4):817-827.
APA	Li, Lulu.,An, Meiling.,Qu, Changfeng.,Zheng, Zhou.,Wang, Yibin.,...&Miao, Jinlai.(2017).Molecular cloning and expression analysis of major intrinsic protein gene in Chlamydomonas sp ICE-L from Antarctica.EXTREMOPHILES,21(4),817-827.
MLA	Li, Lulu,et al."Molecular cloning and expression analysis of major intrinsic protein gene in Chlamydomonas sp ICE-L from Antarctica".EXTREMOPHILES 21.4(2017):817-827.