The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L | |
An, Meiling1; Zheng, Zhou1,2,3; Qu, Changfeng2,3; Wang, Xixi2; Chen, Hao1; Shi, Chongli4; Miao, Jinlai1,2,3,4 | |
刊名 | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS |
2018-05 | |
卷号 | 809页码:13-19 |
关键词 | Chlamydomonas sp ICE-L (6-4) Photolyase DNA damage Photoreactivation |
ISSN号 | 0027-5107 |
DOI | 10.1016/j.mrfmmm.2018.03.004 |
英文摘要 | The psychrophilic microalga, Chlamydomonas sp. ICE-L, isolated from floating ice in the Antarctic, one of the most highly UV exposed ecosystems on Earth, displays an efficient DNA photorepair capacity. Here, the first known (6-4) photolyase gene (6-4CiPhr) from C. sp. ICE-L was identified. The 6-4CiPhr encoded 559-amino acid polypeptide with a pI of 8.86, and had a predicted Mw of 64.2 kDa. Real-time PCR was carried out to investigate the response of 6-4CiPhr to UVB exposure. The transcription of 6-4CiPhr was up-regulated continuously within 6 h, achieving a maximum of 62.7-fold at 6 h. Expressing 6-4CiPhr in a photolyase-deficient Escherichia coli strain improved survival rate of the strain. In vitro activity assays of purified protein demonstrated that 6-4CiPhr was a photolyase with 6-4PP repair activity. These findings improve understanding of photoreactivation mechanisms of (6-4) photolyase. |
资助项目 | Qingdao Entrepreneurship & Innovation Pioneers Program[15-10-3-15-(44)-zch] |
WOS关键词 | DROSOPHILA CRYPTOCHROME ; DIMER REPAIR ; LIGHT ; FAMILY ; PHOTOPRODUCT ; MECHANISMS ; INVITRO ; TIME |
WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000432598800003 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/26496] |
专题 | 自然资源部第一海洋研究所 |
通讯作者 | Miao, Jinlai |
作者单位 | 1.Qingdao Univ, Coll Med, Qingdao 266071, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 3.Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod Qingdao, Qingdao 266237, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China |
推荐引用方式 GB/T 7714 | An, Meiling,Zheng, Zhou,Qu, Changfeng,et al. The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2018,809:13-19. |
APA | An, Meiling.,Zheng, Zhou.,Qu, Changfeng.,Wang, Xixi.,Chen, Hao.,...&Miao, Jinlai.(2018).The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,809,13-19. |
MLA | An, Meiling,et al."The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 809(2018):13-19. |
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