Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater

doi:10.1016/j.chemosphere.2018.02.097

	Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater
	Cui, Zhisong 1; Luan, Xiao 1,2; Jiang, Huichao 3; Li, Qian 1; Xu, Guangfei 1; Sun, Chengjun 1; Zheng, Li 1; Song, Yizhi 4; Davison, Paul A.5; Huang, Wei E.4
刊名	CHEMOSPHERE
	2018-06
卷号	200 页码:322-329
关键词	Cytotoxicity Heavy metal Marine environment Luminescent bacteria test assay Mugilogobius chulae Acinetobacter baylyi
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2018.02.097
英文摘要	toxicity biosensor Acinetobacter baylyi Tox2 was constructed with the host strain A. baylyi ADP1 harboring a new and medium-copy-number plasmid pWH1274_lux, and was applied to detect the cytotoxicity of heavy metal contaminated seawater. The gene cassette luxCDABE was controlled by constitutively expressed promoter P-ret on pWH1274_lux and the bioluminescence intensity of the biosensor reduces in proportional to the concentrations of toxic compounds. A. baylyi Tox2 exhibits tolerance to salinity, hence it is applicable to seawater samples. A. baylyi Tox2 and Mugilogobius chulae were exposed to different concentrations of heavy metals (Hg2+, Zn2+, Cu2+, and Cd2+) in artificial seawater for performance comparison and Pearson correlation analysis showed a significant correlation (p < 0.01) between A. baylyi Tox2 toxicity detection and the fish (M. chulae) exposure test. This suggests that the performance of A. baylyi Tox2 is comparable to the conventional fish toxicity test in terms of cytotoxicity detection of heavy metal contaminated seawater. Furthermore, A. baylyi Tox2 was used to evaluate cytotoxicity of field-collected seawater samples. The results indicate that there was a significant correlation between the luminescence inhibition ratio (IR) of A. baylyi Tox2 and heavy metal concentrations detected by ICP-MS in the samples. Two seawater samples, which contained a high concentration of total heavy metals, exhibited stronger cytotoxicity than the samples containing low concentrations of heavy metals. In conclusion, A. baylyi Tox2 can be used as an alternative tool to aquatic animals for the evaluation of the cytotoxicity of heavy metal contamination in the marine environment. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	NERC in the UK[NE/M002934/1]
WOS关键词	ACUTE TOXICITY ; VIBRIO-FISCHERI ; ACINETOBACTER-BAYLYI ; LUMINESCENT BACTERIA ; WATER HARDNESS ; FISH ; GENOTOXICITY ; BIOREPORTER ; COPPER ; BIOACCUMULATION
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000429891300036
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26277]
专题	自然资源部第一海洋研究所
通讯作者	Cui, Zhisong; Huang, Wei E.
作者单位	1.State Ocean Adm China, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 3.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China 4.Univ Oxford, Dept Engn Sci, Parks Rd, Oxford OX1 3PJ, England 5.Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
推荐引用方式 GB/T 7714	Cui, Zhisong,Luan, Xiao,Jiang, Huichao,et al. Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater[J]. CHEMOSPHERE,2018,200:322-329.
APA	Cui, Zhisong.,Luan, Xiao.,Jiang, Huichao.,Li, Qian.,Xu, Guangfei.,...&Huang, Wei E..(2018).Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater.CHEMOSPHERE,200,322-329.
MLA	Cui, Zhisong,et al."Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater".CHEMOSPHERE 200(2018):322-329.