Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans

doi:10.1007/s00300-014-1508-8

	Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans
	Liu, Shenghao 1,2; Wang, Jing 1; Cong, Bailin 2; Huang, Xiaohang 2; Chen, Kaoshan 1; Zhang, Pengying 1
刊名	POLAR BIOLOGY
	2014-08
卷号	37 期号:8 页码:1145-1155
关键词	Cold adaptation Pohlia nutans HSP70 Psychrophile Abiotic stress
ISSN号	0722-4060
DOI	10.1007/s00300-014-1508-8
英文摘要	Under stress conditions, the mitochondrial respiratory chain generates reactive oxygen species that cause oxidative damage to lipids, proteins and nucleic acids. Mitochondrial chaperones are essential for repairing denatured proteins in plants confronted with diverse stresses. However, their functions have not been well characterized in lower plants such as bryophytes. Here, we cloned a heat-shock 70 protein gene (designated PnHSP70) from the Antarctic moss Pohlia nutans and investigated its transcription levels under stress conditions. The PnHSP70 cDNA encoded a polypeptide of 678 amino acids with an ATPase domain, a substrate peptide-binding domain and a C-terminal domain. Sequence alignment and phylogenetic analysis showed that PnHSP70 was homologous to other known mitochondrial HSP70 proteins. In addition, subcellular localization analysis in which the transient expression of PnHSP70-green fluorescent protein was observed in Arabidopsis thaliana mesophyll protoplasts revealed that PnHSP70 was targeted to mitochondria. Real-time PCR analysis showed that low or high temperature, salinity, drought or PEG treatments as well as UV-B radiation could induce up-regulation of PnHSP70 expression levels. The plant hormones abscisic acid and jasmonic acid also up-regulated the expression levels of PnHSP70. Together, our results revealed that PnHSP70, as a mitochondrial chaperone, might play an important role in the adaptation of the Antarctic moss P. nutans to the polar environment.
资助项目	Chinese Polar Environment Comprehensive Investigation and Assessment Programmes[CHINARE2012-01-06]
WOS关键词	MOLECULAR-CLONING ; HSP70 CHAPERONES ; PROTEIN IMPORT ; SHOCK-PROTEIN ; STRESS ; ACCUMULATION ; TOLERANCE ; ACCLIMATION ; ENVIRONMENT ; SYSTEM
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000341378000008
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26003]
专题	自然资源部第一海洋研究所
通讯作者	Zhang, Pengying
作者单位	1.Shandong Univ, Natl Glycoengn Res Ctr, Coll Life Sci, Jinan 250100, Peoples R China 2.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shenghao,Wang, Jing,Cong, Bailin,et al. Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans[J]. POLAR BIOLOGY,2014,37(8):1145-1155.
APA	Liu, Shenghao,Wang, Jing,Cong, Bailin,Huang, Xiaohang,Chen, Kaoshan,&Zhang, Pengying.(2014).Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans.POLAR BIOLOGY,37(8),1145-1155.
MLA	Liu, Shenghao,et al."Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans".POLAR BIOLOGY 37.8(2014):1145-1155.