PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis

doi:10.1007/s10725-017-0314-z

	PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis
	Li, Chengcheng 1,3; Liu, Shenghao 2; Yao, Xinghao 1,3; Wang, Jing 1; Wang, Tailin 1,3; Zhang, Zhaohui 2; Zhang, Pengying 1,3; Chen, Kaoshan 1,3
刊名	PLANT GROWTH REGULATION
	2017-12
卷号	83 期号:3 页码:489-500
关键词	Antarctic moss Flavonoids Flavanone 3-hydroxylase Phenotype Salt stress
ISSN号	0167-6903
DOI	10.1007/s10725-017-0314-z
英文摘要	Flavonoids are widely distributed secondary metabolites in plants. However, far less research has been carried out on genes involved in the flavonoid biosynthetic pathways in bryophytes. Here, a novel flavanone 3-hydroxylase gene (PnF3H) was cloned from Antarctic moss Pohlia nutans. Subcellular localization revealed that PnF3H were mainly distributed to cytosol. The expression of PnF3H was rapidly induced by salt, UV-B, cold and drought stresses as well as by exogenously applied abscisic acid (ABA). Transgenic Arabidopsis plants of overexpressing PnF3H exhibited enhanced tolerance to salt and oxidative stresses. The expression levels of several stress resistance genes (i.e., AtSOS3, AtP5CS1, AtHKT1, AtCAT1 and AtAPX1) were markedly up-regulated in transgenic plants. The activities of antioxidant enzymes were increased, while the content of hydrogen peroxide was obviously decreased in PnF3H transgenic plants. Meanwhile, the expression levels of ABA-responsive genes which confer for controlling seeds germination, such as AtABI4, AtABI5, AtABI3 and AtABF3 were markedly decreased. Additionally, overexpressing PnF3H alleviated the inhibitory effects of naringenin on plant growth and changed the flavonoid components in transgenic plants. Therefore, we proposed that PnF3H was a flavanone 3-hydroxylase involving in regulating the salinity tolerance and ABA sensitivity to enable P. nutans to adapt to polar climates.
资助项目	Basic Scientific Fund for National Public Research Institutes of China[2014T04]
WOS关键词	RECEPTOR-LIKE KINASE ; GENE-EXPRESSION ; BIOSYNTHETIC-PATHWAY ; OXIDATIVE STRESS ; ABIOTIC STRESS ; OVEREXPRESSION ; PLANT ; IDENTIFICATION ; ACCUMULATION ; FLAVONOIDS
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000414377500013
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25654]
专题	自然资源部第一海洋研究所
通讯作者	Zhang, Pengying
作者单位	1.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Shandong, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China 3.Shandong Prov Key Lab Carbohydrate Chem & Glycobi, Jinan 250100, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Chengcheng,Liu, Shenghao,Yao, Xinghao,et al. PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis[J]. PLANT GROWTH REGULATION,2017,83(3):489-500.
APA	Li, Chengcheng.,Liu, Shenghao.,Yao, Xinghao.,Wang, Jing.,Wang, Tailin.,...&Chen, Kaoshan.(2017).PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis.PLANT GROWTH REGULATION,83(3),489-500.
MLA	Li, Chengcheng,et al."PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis".PLANT GROWTH REGULATION 83.3(2017):489-500.