Analysis of cyclic peptides in pseudostellaria heterophylla (Miq.) pax by high performance liquid Chromatographic/Electrospray ionization time of flight mass Spectrometry

	Analysis of cyclic peptides in pseudostellaria heterophylla (Miq.) pax by high performance liquid Chromatographic/Electrospray ionization time of flight mass Spectrometry
	Han Chao ; Chen Junhui ; Liu Jie ; Wang Xiaoru ; Lee, Frank Sen-Chun
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2006-12
卷号	34 期号:12 页码:1719-1722
关键词	high performance liquid chromatography/electrospray ionization time of flight mass spectrometry pseudostellaria heterophylla ( Miq.) Pax cyclic peptides
ISSN号	0253-3820
英文摘要	A new method for the analysis of cyclic peptides in Pseudostellaria heterophylla ( Miq.) Pax was established by high performance liquid chromatography/electrospray ionization time -of-flight -mass spectrometry (HPLC/ESI-TOFMS). The real samples were separated by a reversed-phase C-18 column using a binary eluent under gradient conditions. Six cyclic peptides were isolated and detected with a diode array detector. The cyclic peptides components were identified with HPLC/ESI-TOF-MS technique. ESI-TOF-MS could offer a higher degree of specificity due to its high resolution, which permits accurate relative molecular mass and fragment ion measurement without lose of sensitivity. The [ M + H] + ions of cyclic peptides in the positive mode were observed for molecule mass information. This method developed has the advantages of simple operation, rapid measurement and accurate determination.
WOS关键词	HIGHER-PLANTS ; OCTAPEPTIDE
WOS研究方向	Chemistry
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000243388100012
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25612]
专题	自然资源部第一海洋研究所
通讯作者	Wang Xiaoru
作者单位	1.Xiamen Univ, Coll Chem & Chem Engn, Minist Educ, Dept Chem, Xiamen 361005, Peoples R China 2.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Analyt Sci, Xiamen 361005, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China 4.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266006, Peoples R China
推荐引用方式 GB/T 7714	Han Chao,Chen Junhui,Liu Jie,et al. Analysis of cyclic peptides in pseudostellaria heterophylla (Miq.) pax by high performance liquid Chromatographic/Electrospray ionization time of flight mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(12):1719-1722.
APA	Han Chao,Chen Junhui,Liu Jie,Wang Xiaoru,&Lee, Frank Sen-Chun.(2006).Analysis of cyclic peptides in pseudostellaria heterophylla (Miq.) pax by high performance liquid Chromatographic/Electrospray ionization time of flight mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(12),1719-1722.
MLA	Han Chao,et al."Analysis of cyclic peptides in pseudostellaria heterophylla (Miq.) pax by high performance liquid Chromatographic/Electrospray ionization time of flight mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.12(2006):1719-1722.