Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans

doi:10.1017/S0954102018000470

	Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans
	Yao, Xinghao 1,2,4; Wang, Tailin 1,2,5; Wang, Huijuan 1,2,4; Liu, Hongwei 1,2,4; Liu, Shenghao 3; Zhao, Qingang 1,2; Chen, Kaoshan 1,2,4; Zhang, Pengying 1,2,4
刊名	ANTARCTIC SCIENCE
	2019-02
卷号	31 期号:1 页码:23-33
关键词	abiotic stress extreme environment flavonoids
ISSN号	0954-1020
DOI	10.1017/S0954102018000470
英文摘要	Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, six genes (Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 and Pn847) were identified in the Antarctic moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence alignment and three-dimensional structure analysis revealed the conserved amino acid residues of the enzymes of the chalcone synthase family, including three catalytic residues (Cys(164), His(303) and Asn(336)) and two substrate recognition residues (Phe(215) and Phe(265)). Phylogenetic analysis indicated that PnCHS088, PnCHS444 and PnCHS768 might be chalcone synthase but that Pn021 is more like stilbenecarboxylate synthase. These genes were located at the transition between fungi and advanced plants in the phylogenetic tree. In addition, real-time PCR analysis revealed that the expression profiles of the six P. nutans genes were influenced by diverse abiotic stresses as well as by abscisic acid and methyl jasmonate. The results presented here contribute to the study of the CHS gene family in polar mosses and further reveal the mechanisms underlying the adaptation of mosses to extreme environments.
资助项目	Basic Scientific Fund for National Public Research Institutes of China[2014T04]
WOS关键词	III POLYKETIDE SYNTHASES ; ARABIDOPSIS-THALIANA ; FLAVONOID BIOSYNTHESIS ; GENETIC DIVERSITY ; STRESS-TOLERANCE ; LOCALIZATION ; SALT ; REGULATOR ; MUTANTS ; CLONING
WOS研究方向	Environmental Sciences & Ecology ; Physical Geography ; Geology
语种	英语
出版者	CAMBRIDGE UNIV PRESS
WOS记录号	WOS:000459031700005
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25426]
专题	自然资源部第一海洋研究所
通讯作者	Zhang, Pengying
作者单位	1.Shandong Univ, Natl Glvcoengn Res Ctr, Jinan 250100, Shandong, Peoples R China 2.Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China 4.Shandong Prov Key Lab Carbohydrate Chem & Glycobi, Jinan 250100, Shandong, Peoples R China 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Yao, Xinghao,Wang, Tailin,Wang, Huijuan,et al. Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans[J]. ANTARCTIC SCIENCE,2019,31(1):23-33.
APA	Yao, Xinghao.,Wang, Tailin.,Wang, Huijuan.,Liu, Hongwei.,Liu, Shenghao.,...&Zhang, Pengying.(2019).Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans.ANTARCTIC SCIENCE,31(1),23-33.
MLA	Yao, Xinghao,et al."Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans".ANTARCTIC SCIENCE 31.1(2019):23-33.