Genetic Variations of the Parasitic Dinoflagellate Hematodinium Infecting Cultured Marine Crustaceans in China

doi:10.1016/j.protis.2016.10.003

	Genetic Variations of the Parasitic Dinoflagellate Hematodinium Infecting Cultured Marine Crustaceans in China
	Xiao, Jie 1; Miao, Xiaoxiang 1; Li, Caiwen 2,3; Xu, Wenjun 4; Zhang, Xuelei 1,3; Wang, Zongling 1
刊名	PROTIST
	2016-12
卷号	167 期号:6 页码:597-609
关键词	Crustacean parasitic dinoflagellate molecular diversity phylogenetic structure ribosome RNA haplotype
ISSN号	1434-4610
DOI	10.1016/j.protis.2016.10.003
英文摘要	The parasitic dinoflagellate Hematodinium infects multiple cultured marine crustaceans and has resulted in significant economic losses to their aquaculture in China. Limited molecular data implied a close relationship among Hematodinium reported in China, whereas the genetic diversity and detailed genetic variation within Hematodinium remains unclear. In order to investigate the genetic diversity and composition of the parasitic dinoflagellate in China, the sequences of the internal transcribed spacer region (ITS1 - 5.8S - ITS2) were amplified from 104 infected hosts cultured in three geographic locations. Two strains were identified, Hematodinium perezi II was the prevalent strain observed in all three host taxa throughout the survey, the other novel one was detected from the single Exopalaemon carinicauda collected from Zhejiang province. Eight ITS haplotypes within H. perezi II were identified with a similarity of 99.8% - 100%. The distinct haplotype compositions and AMOVA analysis suggested a significant genetic differentiation and population structure among the three geographic populations of H. perezi II. The novel strain, genetically affiliated with H. perezi, was quite divergent from the three known genotypes (I, II and III) of H. perezi, and further investigation is needed to determine the distribution and host range of this new strain. (C) 2016 Elsevier GmbH. All rights reserved.
资助项目	Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ01] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS关键词	CRAB CALLINECTES-SAPIDUS ; BLUE-CRAB ; PERKINSUS-MARINUS ; MICROSATELLITE MARKERS ; INTRAGENOMIC VARIATION ; DEVELOPMENTAL CYCLE ; DECAPOD CRUSTACEANS ; MOLECULAR EVIDENCE ; COASTAL WATERS ; SP ALVEOLATA
WOS研究方向	Microbiology
语种	英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
WOS记录号	WOS:000390444900007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25398]
专题	自然资源部第一海洋研究所
通讯作者	Li, Caiwen
作者单位	1.State Ocean Adm, Key Lab Sci & Engn Marine Ecol & Environm, Inst Oceanog 1, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 4.Marine & Fisheries Res Inst Zhejiang Prov, Key Lab Mariculture & Enhancement, Zhoushan 316100, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Jie,Miao, Xiaoxiang,Li, Caiwen,et al. Genetic Variations of the Parasitic Dinoflagellate Hematodinium Infecting Cultured Marine Crustaceans in China[J]. PROTIST,2016,167(6):597-609.
APA	Xiao, Jie,Miao, Xiaoxiang,Li, Caiwen,Xu, Wenjun,Zhang, Xuelei,&Wang, Zongling.(2016).Genetic Variations of the Parasitic Dinoflagellate Hematodinium Infecting Cultured Marine Crustaceans in China.PROTIST,167(6),597-609.
MLA	Xiao, Jie,et al."Genetic Variations of the Parasitic Dinoflagellate Hematodinium Infecting Cultured Marine Crustaceans in China".PROTIST 167.6(2016):597-609.