Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis

	Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis
	Li, Ying Xia 1,2; Wang, Guang Ce 2; Niu, Jian Feng 2; Gao, Zheng Quan ; Chen, Chang Sheng 3,4,5
刊名	INDIAN JOURNAL OF GEO-MARINE SCIENCES
	2013-02
卷号	42 期号:1 页码:11-15
关键词	Porphyra haitanesis thylakoid membranes photosystem II
ISSN号	0379-5136
英文摘要	Thylakoid membranes were isolated and purified from gametophyte of Porphyra haitanesis by sucrose density gradient ultracentrifugation. After P. haitanesis gametophyte thylakoid membranes were solubilized with sodium dodecyl sulfate (SDS), the highly pure and active photosystem (PS) II complex was isolated and characterized by the room absorption and fluorescence emission spectra, DCIP (2, 6-Dichloroindophenol) reduction and oxygen- evolving rates. The PS II complex from P. haitanesis had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 urn) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Acquired PS II complex exhibited high oxygen evolution (879 mu mol O-2. mg(-1) Chl. h(-1)) in the present of 2.5 mM DCIP as an artificial acceptor and was active in photoreduction of DCIP (2, 6- dichloroindophenol) by DPC (1, 5- diphenylcarbazide) at 104 ueq. mg(-1).Chl a. h(-1). Extrinsic proteins of P. haitanesis PS II consisted of 33, 20, 16, 12 kD proteins and cyt c-550 (15 kD), and were different with that of cyanobacteria and higher plants.
资助项目	National Natural Science Foundation of China[U0633006] ; National Natural Science Foundation of China[40476059]
WOS关键词	EXTRINSIC PROTEINS ; THYLAKOID MEMBRANES ; CYANIDIUM-CALDARIUM ; RECONSTITUTION ; POLYPEPTIDES ; YEZOENSIS ; BINDING ; PSII
WOS研究方向	Oceanography
语种	英语
出版者	NATL INST SCIENCE COMMUNICATION-NISCAIR
WOS记录号	WOS:000317453500002
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25219]
专题	自然资源部第一海洋研究所
通讯作者	Li, Ying Xia
作者单位	1.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Nation Deep Sea Ctr State Ocean Adm, Qingdao, Peoples R China 4.Shandong Univ Technol, Sch Life Sci, Zibo, Peoples R China 5.Jimei Univ, Dept Aquaculture, Inst Fishery Biotechnol, Xiamen 361021, Peoples R China
推荐引用方式 GB/T 7714	Li, Ying Xia,Wang, Guang Ce,Niu, Jian Feng,et al. Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(1):11-15.
APA	Li, Ying Xia,Wang, Guang Ce,Niu, Jian Feng,Gao, Zheng Quan,&Chen, Chang Sheng.(2013).Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis.INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(1),11-15.
MLA	Li, Ying Xia,et al."Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.1(2013):11-15.