Characterization of cupric glutamate extinguishing mechanism of Alexandrium sp LC3 with two-dimensional electrophoresis and MALDI-TOF MS

doi:10.1007/s10126-008-9091-z

	Characterization of cupric glutamate extinguishing mechanism of Alexandrium sp LC3 with two-dimensional electrophoresis and MALDI-TOF MS
	Li, Hao 1,2; Miao, Jinlai 1; Cui, Fengxia 3; Li, Guangyou 1
刊名	MARINE BIOTECHNOLOGY
	2008-09
卷号	10 期号:5 页码:527-537
关键词	Alexandrium sp LC3 cupric glutamate extinguish plasma membrane permeability two-dimensional electrophoresis matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry
ISSN号	1436-2228
DOI	10.1007/s10126-008-9091-z
英文摘要	Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth.
WOS关键词	RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE ; HARMFUL ALGAL BLOOMS ; GEL-ELECTROPHORESIS ; OXIDATIVE STRESS ; LARGE SUBUNIT ; RED TIDE ; PROTEINS ; MEMBRANE ; COPPER ; ZINC
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000258323900007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/24975]
专题	自然资源部第一海洋研究所
通讯作者	Miao, Jinlai
作者单位	1.SOA, Key Lab Marine Biol Act Subst, Qingdao 266061, Peoples R China 2.Ocean Univ China, Marine Life Sci Coll, Qingdao 266003, Peoples R China 3.China Natl Res Inst Food & Fermentat Ind, Beijing 100037, Peoples R China
推荐引用方式 GB/T 7714	Li, Hao,Miao, Jinlai,Cui, Fengxia,et al. Characterization of cupric glutamate extinguishing mechanism of Alexandrium sp LC3 with two-dimensional electrophoresis and MALDI-TOF MS[J]. MARINE BIOTECHNOLOGY,2008,10(5):527-537.
APA	Li, Hao,Miao, Jinlai,Cui, Fengxia,&Li, Guangyou.(2008).Characterization of cupric glutamate extinguishing mechanism of Alexandrium sp LC3 with two-dimensional electrophoresis and MALDI-TOF MS.MARINE BIOTECHNOLOGY,10(5),527-537.
MLA	Li, Hao,et al."Characterization of cupric glutamate extinguishing mechanism of Alexandrium sp LC3 with two-dimensional electrophoresis and MALDI-TOF MS".MARINE BIOTECHNOLOGY 10.5(2008):527-537.