Lipopeptide biosurfactant production bacteria Acinetobacter sp D3-2 and its biodegradation of crude oil

doi:10.1039/c3em00600j

	Lipopeptide biosurfactant production bacteria Acinetobacter sp D3-2 and its biodegradation of crude oil
	Bao, Mutai 1; Pi, Yongrui 1; Wang, Lina 1; Sun, Peiyan 2; Li, Yiming 1; Cao, Lixin 2
刊名	ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
	2014
卷号	16 期号:4 页码:897-903
ISSN号	2050-7887
DOI	10.1039/c3em00600j
英文摘要	In this work, a hydrocarbon-degrading bacterium D3-2 isolated from petroleum contaminated soil samples was investigated for its potential effect in biodegradation of crude oil. The strain was identified as Acinetobacter sp. D3-2 based on morphological, biochemical and phylogenetic analysis. The optimum environmental conditions for growth of the bacteria were determined to be pH 8.0, with a NaCl concentration of 3.0% (w/v) at 30 degrees C. Acinetobacter sp. D3-2 could utilize various hydrocarbon substrates as the sole carbon and energy source. From this study, we also found that the strain had the ability to produce biosurfactant, with the production of 0.52 g L-1. The surface tension of the culture broth was decreased from 48.02 to 26.30 mN m(-1). The biosurfactant was determined to contain lipopeptide compounds based on laboratory analyses. By carrying out a crude oil degradation assay in an Erlenmeyer flask experiment and analyzing the hydrocarbon removal rate using gas chromatography, we found that Acinetobacter sp. D3-2 could grow at 30 degrees C in 3% NaCl solution with a preferable ability to degrade 82% hydrocarbons, showing that bioremediation does occur and plays a profound role during the oil reparation process.
资助项目	Open Foundation of Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology of SOA[201104]
WOS关键词	MARINE ; BIOREMEDIATION ; STRAINS ; GROWTH ; SEA
WOS研究方向	Chemistry ; Environmental Sciences & Ecology
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000333565000024
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/24944]
专题	自然资源部第一海洋研究所
通讯作者	Bao, Mutai
作者单位	1.Ocean Univ China, Key Lab Marine Chem Theory & Technol, Minist Educ, Qingdao 266100, Shandong, Peoples R China 2.State Ocean Adm, North China Sea Environm Monitoring Ctr, Key Lab Marine Spill Oil Identificat & Damage Ass, Qingdao 266033, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Bao, Mutai,Pi, Yongrui,Wang, Lina,et al. Lipopeptide biosurfactant production bacteria Acinetobacter sp D3-2 and its biodegradation of crude oil[J]. ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS,2014,16(4):897-903.
APA	Bao, Mutai,Pi, Yongrui,Wang, Lina,Sun, Peiyan,Li, Yiming,&Cao, Lixin.(2014).Lipopeptide biosurfactant production bacteria Acinetobacter sp D3-2 and its biodegradation of crude oil.ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS,16(4),897-903.
MLA	Bao, Mutai,et al."Lipopeptide biosurfactant production bacteria Acinetobacter sp D3-2 and its biodegradation of crude oil".ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS 16.4(2014):897-903.