Separation and Identification of Metallothionein Isoforms and Sub-isoforms by Liquid Chromatography

	Separation and Identification of Metallothionein Isoforms and Sub-isoforms by Liquid Chromatography
	Lu Suge 1,2; Shen Jincan 1,2; Zhuang Zhixia 1,2,3; Wang Xiaoru 1,2,3
刊名	CHINESE JOURNAL OF CHROMATOGRAPHY
	2005-03
卷号	23 期号:2 页码:164-167
关键词	ion-exchange chromatography gel exclusion chromatography reversed-phase high performance liquid chromatography metallothioneins isoforms
ISSN号	1000-8713
英文摘要	A method has been established for the separation and identification of metallothionein (MT) isoforms and sub-isoforms by liquid chromatography. A mixture of rabbit liver MT was separated on a DEAE A-25 weak anion-exchange chromatographic (AEC) column. Inductively coupled plasma-mass spectrometer (ICP-MS) was used for off-line detection of Zn in each fraction. The two main MT isoforms, MT-1 and MT-2, were isolated. The collected MT isoform fractions were then desalted by a homemade Sephadex G-25 size exclusion chromatographic (SEC) column. Afterwards, the sample was separated on a C18 reversed-phase column with UV detection at 210 nm. Different separation conditions were discussed and several subisoforms of MT were well separated at pH 2. 0. The sub-isoforms were finally characterized by on-line HPLC-ESI-MS. The isoforms and sub-isoforms were well separated on a reversed-phase column under optimized chromatographic conditions and the detection results of ESI-MS were in agreement with the data found in literature. The method developed can be well used for the separation of metallothionein isoforms and sub-isoforms.
WOS研究方向	Chemistry
语种	中文
出版者	SCIENCE PRESS
WOS记录号	WOS:000435235100010
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/21461]
专题	自然资源部第一海洋研究所
通讯作者	Lu Suge
作者单位	1.Xiamen Univ, Coll Chem & Chem Engn, Minist Educ, Dept Chem, Xiamen 361005, Peoples R China 2.Xiamen Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Analyt Sci, Xiamen 361005, Peoples R China 3.State Ocean Adm, Qingdao Key Lab Analyt Technol Dev & Standardizat, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Lu Suge,Shen Jincan,Zhuang Zhixia,et al. Separation and Identification of Metallothionein Isoforms and Sub-isoforms by Liquid Chromatography[J]. CHINESE JOURNAL OF CHROMATOGRAPHY,2005,23(2):164-167.
APA	Lu Suge,Shen Jincan,Zhuang Zhixia,&Wang Xiaoru.(2005).Separation and Identification of Metallothionein Isoforms and Sub-isoforms by Liquid Chromatography.CHINESE JOURNAL OF CHROMATOGRAPHY,23(2),164-167.
MLA	Lu Suge,et al."Separation and Identification of Metallothionein Isoforms and Sub-isoforms by Liquid Chromatography".CHINESE JOURNAL OF CHROMATOGRAPHY 23.2(2005):164-167.