Involvement of VAMP-2 in exocytosis of IL-1 beta in turbot (Scophthalmus maximus) leukocytes after Vibrio anguillarum infection

doi:10.1016/j.bbrc.2006.01.138

	Involvement of VAMP-2 in exocytosis of IL-1 beta in turbot (Scophthalmus maximus) leukocytes after Vibrio anguillarum infection
	Chai, YM ; Huang, XH ; Cong, BL ; Liu, SH ; Chen, K ; Li, GY ; Gaisano, HY
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2006-04-07
卷号	342 期号:2 页码:509-513
关键词	IL-1 beta VAMP-2 turbot leukocytes exocytosis
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2006.01.138
英文摘要	Vibrio anguillarum is a major pathogen threatening the fish aquaculture in China. Infection of cultivated turbot (Scophthalmus maximus) with V. anguillarum induced rapid synthesis and secretion of IL-1 beta, which initiates the innate immune response. SNARE proteins are known to regulate vesicular trafficking and fusion in all eukaryotes. Here, we determined whether SNARE proteins, specifically vesicle-associated membrane protein-2 (VAMP-2), are involved in regulated exocytosis of IL-1 beta of leukocytes in marine fish. We show that VAMP-2 is present in turbot blood leukocytes, with nucleotide sequence identity of 88.2% and 93.0% to those of zebra fish and sea bass, respectively. After Vibrio infection, turbot leukocyte VAMP-2 was increased at the levels of transcription and translation in a temporal pattern coinciding with leukocyte IL-1 beta secretion. Confocal microscopy localized VAMP-2 to vesicle structures in leukocytes. Taken together, our results suggest that VAMP-2 is involved in regulated exocytosis of cytokines in immunocytes in fish. (c) 2006 Elsevier Inc. All rights reserved.
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000236014500019
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4676]
专题	自然资源部第一海洋研究所
作者单位	1.SOA, Marine Bioact Subst Lab, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Ocean Univ China, Dept Life Sci, Qingdao 266003, Peoples R China; 3.Shandong Univ, Coll Marine Life Sci, Weihai 264209, Peoples R China; 4.Univ Toronto, Dept Med, Toronto, ON M5S 1A8, Canada
推荐引用方式 GB/T 7714	Chai, YM,Huang, XH,Cong, BL,et al. Involvement of VAMP-2 in exocytosis of IL-1 beta in turbot (Scophthalmus maximus) leukocytes after Vibrio anguillarum infection[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,342(2):509-513.
APA	Chai, YM.,Huang, XH.,Cong, BL.,Liu, SH.,Chen, K.,...&Gaisano, HY.(2006).Involvement of VAMP-2 in exocytosis of IL-1 beta in turbot (Scophthalmus maximus) leukocytes after Vibrio anguillarum infection.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,342(2),509-513.
MLA	Chai, YM,et al."Involvement of VAMP-2 in exocytosis of IL-1 beta in turbot (Scophthalmus maximus) leukocytes after Vibrio anguillarum infection".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 342.2(2006):509-513.