Dual effect of inorganic polymeric phosphate/polyphosphate on;osteoblasts and osteoclasts in vitro

doi:10.1002/term.1465

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	Dual effect of inorganic polymeric phosphate/polyphosphate on;osteoblasts and osteoclasts in vitro
	Wang, Xiaohong 4; Schroeder, Heinz C.; Diehl-Seifert, Baerbel ; Kropf, Klaus ; Schlossmacher, Ute ; Wiens, Matthias ; Mueller, Werner E. G.
刊名	JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
	2013
卷号	7 期号:10 页码:767-776
关键词	inorganic polymeric phosphate polyphosphate osteoblasts osteoclasts SaOS-2 cells RAW 264.7 cells hydroxyapatite formation osteoblastogenesis osteoclastogenesis
ISSN号	1932-6254
DOI	10.1002/term.1465
英文摘要	Inorganic polymeric phosphate/polyphosphate (polyP) is a natural polymer existing in both pro- and eukaryotic systems. In the present study the effect of polyP as well as of polyP supplied in a stoichiometric ratio of 2m polyP:1m CaCl2 [polyP (Ca2+ complex)] on the osteoblast-like SaOS-2 cells and the osteoclast-like RAW 264.7 cells was determined. Both polymers are non-toxic for these cells up to a concentration of 100 mu m. In contrast to polyP, polyP (Ca2+ complex) significantly induced hydroxyapatite formation at a concentration >10 mu m, as documented by alizarin red S staining and scanning electron microscopic (SEM) inspection. Furthermore, polyP (Ca2+ complex) triggered in SaOS-2 cells transcription of BMP2 (bone morphogenetic protein 2), a cytokine involved in maturation of hydroxyapatite-forming cells. An additional activity of polyP (Ca2+ complex) is described by showing that this polymer impairs osteoclastogenesis. At concentrations>10 mu m polyP (Ca2+ complex) slows down the progression of RAW 264.7 cells to functional osteoclasts, as measured by the expression of TRAP (tartrate-resistant acid phosphatase). Finally, it is shown that 10-100 mu m polyP (Ca2+ complex) inhibited phosphorylation of IB by the respective kinase in RAW 264.7 cells. We concluded that polyP (Ca2+ complex) displays a dual effect on bone metabolizing cells. It promotes hydroxyapatite formation in SaOS-2 cells (osteoblasts) and impairs maturation of the osteoclast-related RAW 264.7 cells. Copyright (c) 2012 John Wiley & Sons, Ltd.
学科主题	其它
WOS关键词	FACTOR-KAPPA-B ; RECEPTOR ACTIVATOR ; SAOS-2 CELLS ; BETA-GLYCEROPHOSPHATE ; GENE-EXPRESSION ; BONE ; POLYPHOSPHATE ; DIFFERENTIATION ; MINERALIZATION ; INHIBITION
WOS研究方向	Cell Biology ; Biotechnology & Applied Microbiology ; Engineering
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000325466300002
内容类型	期刊论文
源URL	[http://119.78.100.197/handle/2HKVOGP0/40061]
专题	中国地质科学院国家地质实验测试中心
作者单位	1.Chinese Acad Geol Sci, Natl Res Ctr Geoanal, Beijing, Peoples R China. 2.Wang, Xiaohong; Schroeder, Heinz C.; Schlossmacher, Ute; Wiens, Matthias; Mueller, Werner E. G.] Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Univ Med Ctr, ERC Adv Investigator Grant Res Grp, D-55128 Mainz, Germany. 3.Schroeder, Heinz C.; Diehl-Seifert, Baerbel; Kropf, Klaus; Wiens, Matthias; Mueller, Werner E. G.] NanotecMARIN GmbH, Mainz, Germany. 4.中国地质科学院国家地质实验测试中心
推荐引用方式 GB/T 7714	Wang, Xiaohong,Schroeder, Heinz C.,Diehl-Seifert, Baerbel,et al. Dual effect of inorganic polymeric phosphate/polyphosphate on;osteoblasts and osteoclasts in vitro[J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2013,7(10):767-776.
APA	Wang, Xiaohong.,Schroeder, Heinz C..,Diehl-Seifert, Baerbel.,Kropf, Klaus.,Schlossmacher, Ute.,...&Mueller, Werner E. G..(2013).Dual effect of inorganic polymeric phosphate/polyphosphate on;osteoblasts and osteoclasts in vitro.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,7(10),767-776.
MLA	Wang, Xiaohong,et al."Dual effect of inorganic polymeric phosphate/polyphosphate on;osteoblasts and osteoclasts in vitro".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 7.10(2013):767-776.