Proteomic profile of mouse fibroblasts exposed to pure magnesium extract

doi:10.1016/j.msec.2016.06.100

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	Proteomic profile of mouse fibroblasts exposed to pure magnesium extract
	Zhen, Zhen ; Luthringer, Berengere ; Yang, Li ; Xi, Tingfei ; Zheng, Yufeng ; Feyerabend, Frank ; Willumeit, Regine ; Lai, Chen ; Ge, Zigang
刊名	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2016
关键词	Proteornics Magnesium Mouse fibroblasts Biodegradable material MAMMALIAN-CELL-CYCLE IN-VITRO FOCAL ADHESIONS OXIDATIVE-PHOSPHORYLATION BIODEGRADABLE MAGNESIUM MEDIATED ENDOCYTOSIS MECHANISMS MODEL PROLIFERATION EXPRESSION
DOI	10.1016/j.msec.2016.06.100
英文摘要	Magnesium and its alloys gain wide attention as degradable biomaterials. In order to reveal the molecular mechanism of the influence of biodegradable magnesium on cells, proteomics analysis was performed in this work. After mouse fibroblasts (L929) were cultured with or without Mg degradation products (Mg-extract) for 8, 24, and 48 h, changes inprotein expression profiles were obtained using isobaric tags for relative and absolute quantitation (iTRAQ) coupled two dimensional liquid chromatography-tandem mass spectrometry (2D LC MS/MS). A total of 867 proteins were identified (relying on at least two peptides). Compared to the control group, 205, 282, and 217 regulated proteins were identified at 8, 24, and 48 h, respectively. 65 common proteins were up or down-regulated within all the three time points, which were involved in various physiological and metabolic activities. Consistent with viability, proliferation, and cell cycle analysis, stimulated energy metabolism as well as protein synthesis pathways were discussed, indicating a possible effect of Mg-extract on L929 proliferation. Furthermore, endocytosis and focal adhesion processes were also discussed. This proteomics study uncovers early cellular mechanisms triggered by Mg degradation products and highlights the cytocompatibility of biodegradable metallic materials for biomedical applications such as stents or orthopaedic implants. (C) 2016 Published by Elsevier B.V.; National Basic Research Program of China [2012CB933600, 2012CB619102]; National Natural Science Foundation of China [31370956]; China Scholarship Council support [20130610156]; Shenzhen Technology Innovation Plan [CXZZ20140731091722497]; Strategic New Industry Development Special Foundation of Shenzhen [JCYJ20140419114548513]; SCI(E); EI; PubMed; ARTICLE; berengere.luthringer@hzg.de; xitingfei@pku.edu.cn; 522-531; 69
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492946]
专题	工学院
推荐引用方式 GB/T 7714	Zhen, Zhen,Luthringer, Berengere,Yang, Li,et al. Proteomic profile of mouse fibroblasts exposed to pure magnesium extract[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2016.
APA	Zhen, Zhen.,Luthringer, Berengere.,Yang, Li.,Xi, Tingfei.,Zheng, Yufeng.,...&Ge, Zigang.(2016).Proteomic profile of mouse fibroblasts exposed to pure magnesium extract.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS.
MLA	Zhen, Zhen,et al."Proteomic profile of mouse fibroblasts exposed to pure magnesium extract".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (2016).