Investigating the potential of multiwalled carbon nanotubes based zinc   nanocomposite as a recognition interface towards plant pathogen   detection

doi:10.1016/j.jviromet.2017.09.004

CORC > 北京大学 > 工学院

	Investigating the potential of multiwalled carbon nanotubes based zinc nanocomposite as a recognition interface towards plant pathogen detection
	Tahir, Muhammad Ali ; Hameed, Sadaf ; Munawar, Anam ; Amin, Imran ; Mansoor, Shahid ; Khan, Waheed S. ; Bajwa, Sadia Zafar
刊名	JOURNAL OF VIROLOGICAL METHODS
	2017
关键词	Multiwalled carbon nanotubes Zinc nanocomposite DNA biosensor Chili leaf curl betasatellite Hybridization ELECTROCHEMICAL DNA SENSOR MONOPARTITE BEGOMOVIRUSES GEL-ELECTROPHORESIS BIOSENSOR ELECTRODE VIRUS ACID BETA FABRICATION SEQUENCES
DOI	10.1016/j.jviromet.2017.09.004
英文摘要	The emergence of nanotechnology has opened new horizons for constructing efficient recognition interfaces. This is the first report where the potential of a multiwalled carbon nanotube based zinc nanocomposite (MWCNTs-Zn NPs) investigated for the detection of an agricultural pathogen i.e. Chili leaf curl betasatellite (ChLCB). Atomic force microscope analyses revealed the presence of multiwalled carbon nanotubes (MWCNTs) having a diameter of 50-100 nm with zinc nanoparticles (Zn-NPs) of 25-500 nm. In this system, these bunches of Zn-NPs anchored along the whole lengths of MWCNTs were used for the immobilization of probe DNA strands. The electrochemical performance of DNA biosensor was assessed in the absence and presence of the complementary DNA during cyclic and differential pulse voltammetry scans. Target binding events occurring on the interface surface patterned with single-stranded DNA was quantitatively translated into electrochemical signals due to hybridization process. In the presence of complementary target DNA, as the result of duplex formation, there was a decrease in the peak current from 1.89 x 10(-04) to 5.84 x 10(-05) A. The specificity of this electrochemical DNA biosensor was found to be three times as compared to non-complementary DNA. This material structuring technique can be extended to design interfaces for the recognition of the other plant viruses and biomolecules.; International Foundation for Science (IFS), Sweden; Organization of Islamic Cooperation's (OIC) Standing Committee on Scientific and Technological Cooperation (COMSTECH) [E-5659]; SCI(E); ARTICLE; 130-136; 249
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/484563]
专题	工学院
推荐引用方式 GB/T 7714	Tahir, Muhammad Ali,Hameed, Sadaf,Munawar, Anam,et al. Investigating the potential of multiwalled carbon nanotubes based zinc nanocomposite as a recognition interface towards plant pathogen detection[J]. JOURNAL OF VIROLOGICAL METHODS,2017.
APA	Tahir, Muhammad Ali.,Hameed, Sadaf.,Munawar, Anam.,Amin, Imran.,Mansoor, Shahid.,...&Bajwa, Sadia Zafar.(2017).Investigating the potential of multiwalled carbon nanotubes based zinc nanocomposite as a recognition interface towards plant pathogen detection.JOURNAL OF VIROLOGICAL METHODS.
MLA	Tahir, Muhammad Ali,et al."Investigating the potential of multiwalled carbon nanotubes based zinc nanocomposite as a recognition interface towards plant pathogen detection".JOURNAL OF VIROLOGICAL METHODS (2017).