A novel stochastic reaction-diffusion model of Ca2+ blink in cardiac   myocytes

doi:10.1016/j.scib.2016.12.001

CORC > 北京大学 > 工学院

	A novel stochastic reaction-diffusion model of Ca2+ blink in cardiac myocytes
	Li, Jinghui ; Xie, Wenjun ; Chen, Xi ; Huo, Yunlong ; Cheng, Heping ; Tan, Wenchang
刊名	SCIENCE BULLETIN
	2017
关键词	stochastic reaction-diffusion model SARCOPLASMIC-RETICULUM SPARKS TERMINATION RELEASE STORE
DOI	10.1016/j.scib.2016.12.001
英文摘要	In cardiac myocytes, the sarcoplasmic reticulum (SR) is the main storage organelle of free Ca~(2+). The concentration of free Ca~(2+) in the SR is 0.5–1.0 mmol/L and is 2–3 orders of magnitude greater than that in the cytosol. The SR is composed of interconnected cisternae (junctional SR, i.e., JSR) and tubules (free SR network, i.e., FSR) that extend throughout the cytosol [1]. Ca~(2+) is released from the JSR into the cytosol via Ca~(2+) release units (CRUs, comprised of several ryanodine receptors – RyRs located in the JSR membrane), resulting in Ca~(2+) sparks and Ca~(2+) blinks which are two views of the same elementary release event, from the standpoints of the cytosol and the SR, respectively. Then the released Ca~(2+) are transported back into the FSR network by SR Ca~(2+)-ATPase (SERCA) pumps located on the FSR network. Ca~(2+) in the FSR network diffuse rapidly into the JSR, getting ready for the next Ca~(2+) release event. It is generally known that a Ca~(2+) spark arises when the clustered RyRs open. As the mirror events of Ca~(2+) sparks in the cytosol, Ca~(2+) blinks represent the Ca~(2+) depletion events in the JSR. Ca~(2+) blinks play an important role in the termination of Ca~(2+) sparks and account for most of the cytosolic Ca~(2+)-transient required to activate the myofilaments [2]. Another type of high-frequency but low-flux quarky Ca~(2+) release events was reported as Ca~(2+) quarks [3]. Ca~(2+) quarks may arise from ‘‘rogue RyRs” which are one or a few RyRs physically uncoupled from the RyR cluster. Ca~(2+) quarks contribute to a phenomenon referred to as ‘‘invisible Ca~(2+) leak” in health and disease, which plays important roles in heart failure. Ca~(2+) quarks may be responsible for Ca~(2+) overload due to its high-frequency. As the mirror events of Ca~(2+) quarks in the cytosol, the quarky Ca~(2+) depletion events in the JSR, which are called as mini Ca~(2+) blinks, are as important as regular Ca~(2+) blinks and needed to research. The local dynamics of SR Ca~(2+), determined by RyR release Ca~(2+) flux and FSR uptake Ca~(2+) flux, has already been visualized experimentally through the recording of regular Ca~(2+) blinks [1,4] and mini Ca~(2+) blinks [3]. However, the experiments could not show the detailed spatiotemporal dynamics of Ca~(2+) blinks because it is nano-scale Ca~(2+) signaling. This can be achieved by theoretical modelling and numerical simulation of Ca~(2+) blinks. Here, taking into consideration the existence of rogue RyRs, we developed a stochastic, spatially-detailed mathematical model for Ca~(2+) blinks in a JSR based on the stochastic gating of rogue and clustered RyRs.; National Natural Science Foundation of China [11272014, 11328201]; National Key Basic Research Program of China [2013CB531200]; SCI(E); 中国科学引文数据库(CSCD); EDITORIAL MATERIAL; 1; 5-8; 62
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/475679]
专题	工学院
推荐引用方式 GB/T 7714	Li, Jinghui,Xie, Wenjun,Chen, Xi,et al. A novel stochastic reaction-diffusion model of Ca2+ blink in cardiac myocytes[J]. SCIENCE BULLETIN,2017.
APA	Li, Jinghui,Xie, Wenjun,Chen, Xi,Huo, Yunlong,Cheng, Heping,&Tan, Wenchang.(2017).A novel stochastic reaction-diffusion model of Ca2+ blink in cardiac myocytes.SCIENCE BULLETIN.
MLA	Li, Jinghui,et al."A novel stochastic reaction-diffusion model of Ca2+ blink in cardiac myocytes".SCIENCE BULLETIN (2017).