Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad   of Diabetic Mice

doi:10.3791/54995

CORC > 北京大学 > 工学院

	Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
	Wang, Kai ; Wang, Xi ; Han, Cheng-sheng ; Chen, Liang-yi ; Luo, Ying
刊名	JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
	2017
关键词	Bioengineering Issue 125 islet isolation scaffold transplantation epididymal fat pad murine diabetes POLYMER SCAFFOLDS LANGERHANS PROTOCOL PANCREAS MOUSE SITE
DOI	10.3791/54995
英文摘要	Islet transplantation has been clinically proven to be effective at treating type 1 diabetes. However, the current intrahepatic transplantation strategy may incur acute whole blood reactions and result in poor islet engraftment. Here, we report a robust protocol for the transplantation of islets at the extrahepatic transplantation site-the epididymal fat pad (EFP)-in a diabetic mouse model. A protocol to isolate and purify islets at high yields from C57BL/6J mice is described, as well as a transplantation method performed by seeding islets onto a decellularized scaffold (DCS) and implanting them at the EFP site in syngeneic C57BL/6J mice rendered diabetic by streptozotocin. The DCS graft containing 500 islets reversed the hyperglycemic condition within 10 days, while the free islets without DCS required at least 30 days. The normoglycemia was maintained for up to 3 months until the graft was explanted. In conclusion, DCS enhanced the engraftment of islets into the extrahepatic site of the EFP, which could easily be retrieved and might provide a reproducible and useful platform for investigating the scaffold materials, as well as other transplantation parameters required for a successful islet engraftment.; National Natural Science Foundation of China [31322021]; SCI(E); ARTICLE; 125
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472297]
专题	工学院
推荐引用方式 GB/T 7714	Wang, Kai,Wang, Xi,Han, Cheng-sheng,et al. Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2017.
APA	Wang, Kai,Wang, Xi,Han, Cheng-sheng,Chen, Liang-yi,&Luo, Ying.(2017).Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS.
MLA	Wang, Kai,et al."Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2017).