Laser-Sintered Constructs with Bio-inspired Porosity and Surface   Micro/Nano-Roughness Enhance Mesenchymal Stem Cell Differentiation and   Matrix Mineralization In Vitro

doi:10.1007/s00223-016-0184-9

CORC > 北京大学 > 工学院

	Laser-Sintered Constructs with Bio-inspired Porosity and Surface Micro/Nano-Roughness Enhance Mesenchymal Stem Cell Differentiation and Matrix Mineralization In Vitro
	Cheng, Alice ; Cohen, David J. ; Boyan, Barbara D. ; Schwartz, Zvi
刊名	CALCIFIED TISSUE INTERNATIONAL
	2016
关键词	Additive manufacturing Stem cells Bone regeneration Orthopedic implants Dental implants OSTEOBLAST LINEAGE CELLS CHEMICALLY TREATED TITANIUM POROUS TITANIUM OSTEOGENIC DIFFERENTIATION ALKALINE-PHOSPHATASE BONE-FORMATION IMPLANTS SCAFFOLDS SCALE OSSEOINTEGRATION
DOI	10.1007/s00223-016-0184-9
英文摘要	Direct metal laser sintering can produce porous Ti-6Al-4V orthopedic and dental implants. The process requires reduced resources and time and can provide greater structural control than machine manufacturing. Implants in bone are colonized by mesenchymal stem cells (MSCs), which can differentiate into osteoblasts and contribute to osseointegration. This study examined osteoblast differentiation and matrix mineralization of human MSCs cultured on laser-sintered Ti-6Al-4V constructs with varying porosity and at different time scales. 2D solid disks and low, medium and high porosity (LP, MP, and HP) 3D constructs based on a human trabecular bone template were laser sintered from Ti-6Al-4V powder and further processed to have micro- and nanoscale roughness. hMSCs exhibited greater osteoblastic differentiation and local factor production on all 3D porous constructs compared to 2D surfaces, which was sustained for 9 days without use of exogenous factors. hMSCs cultured for 8 weeks on MP constructs in osteogenic medium (OM), OM supplemented with BMP2 or collagen-coated MP constructs in OM exhibited bone-like extracellular matrix mineralization. Use of bio-inspired porosity for the 3D architecture of additively manufactured Ti-6Al-4V enhanced osteogenic differentiation of hMSCs beyond surface roughness alone. This study suggests that a 3D architecture may enhance the osseointegration of orthopedic and dental implants in vivo.; AB Dental; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [AR052102]; SCI(E); ARTICLE; bboyan@vcu.edu; 6; 625-637; 99
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/458289]
专题	工学院
推荐引用方式 GB/T 7714	Cheng, Alice,Cohen, David J.,Boyan, Barbara D.,et al. Laser-Sintered Constructs with Bio-inspired Porosity and Surface Micro/Nano-Roughness Enhance Mesenchymal Stem Cell Differentiation and Matrix Mineralization In Vitro[J]. CALCIFIED TISSUE INTERNATIONAL,2016.
APA	Cheng, Alice,Cohen, David J.,Boyan, Barbara D.,&Schwartz, Zvi.(2016).Laser-Sintered Constructs with Bio-inspired Porosity and Surface Micro/Nano-Roughness Enhance Mesenchymal Stem Cell Differentiation and Matrix Mineralization In Vitro.CALCIFIED TISSUE INTERNATIONAL.
MLA	Cheng, Alice,et al."Laser-Sintered Constructs with Bio-inspired Porosity and Surface Micro/Nano-Roughness Enhance Mesenchymal Stem Cell Differentiation and Matrix Mineralization In Vitro".CALCIFIED TISSUE INTERNATIONAL (2016).