Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week   Root Canal Enterococcus faecalis Biofilm In Vitro

doi:10.1016/j.joen.2014.10.020

CORC > 北京大学 > 工学院

	Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro
	Li, Yinglong ; Sun, Ke ; Ye, Guopin ; Liang, Yongdong ; Pan, Hong ; Wang, Guomin ; Zhao, Yijiao ; Pan, Jie ; Zhang, Jue ; Fang, Jing
刊名	JOURNAL OF ENDODONTICS
	2015
关键词	Cold plasma Enterococcus faecalis biofilm mechanical safety microhardness roughness 2-PERCENT CHLORHEXIDINE GEL CALCIUM HYDROXIDE ANTIMICROBIAL EFFICACY BACTERIAL BIOFILMS SUSCEPTIBILITY INFECTION MODEL INSTRUMENTATION RESISTANCE TRIAL
DOI	10.1016/j.joen.2014.10.020
英文摘要	Introduction: Although endodontic infection is caused by multi-bacteria species, Enterococcus faecalis is usually isolated in chronic apical periodontitis. The aim of this study was to evaluate the effectiveness and mechanical safety of cold plasma therapy in disinfecting 3-week E. faecalis biofilms. Methods: Teeth with 3-week E. faecalis biofilm were treated with AC argon/oxygen (Ar/O-2) cold plasma for various treatment times and compared with those treated with Ca(OH)(2), 2% chlorhexidine gel, and Ca(OH)(2)/chlorhexidine for a week. Antimicrobial efficacy was assessed by colony-forming unit method. Scanning electron microscopy was used to assess the morphologic changes of E. faecalis biofilm by plasma. Confocal laser scanning microscopy was used to confirm the viability of the biofilm after the plasma treatment. Microhardness and roughness changes of root canal dentin caused by plasma were verified with Vickers Hardness Tester and 3D Profile Measurement Laser Microscope, respectively. Results: There were no detectable live bacteria after 12 minutes of cold plasma treatment. This was further confirmed by scanning electron microscopy and confocal laser scanning microscopy results. Microhardness and roughness of root canal dentin showed no significant difference after plasma treatment. Conclusions: Atmospheric pressure cold plasma is an effective therapy in endodontics for its strong sterilization effect on fully matured biofilm within a few minutes. Meanwhile, it has art accepted mechanical safety for its low temperature and not affecting the microhardness and roughness of root canal dentin significantly.; 985 program of Peking University; SCI(E); PubMed; ARTICLE; panjie72@sina.com; 8; 1325-1330; 41
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417702]
专题	工学院
推荐引用方式 GB/T 7714	Li, Yinglong,Sun, Ke,Ye, Guopin,et al. Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro[J]. JOURNAL OF ENDODONTICS,2015.
APA	Li, Yinglong.,Sun, Ke.,Ye, Guopin.,Liang, Yongdong.,Pan, Hong.,...&Fang, Jing.(2015).Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro.JOURNAL OF ENDODONTICS.
MLA	Li, Yinglong,et al."Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro".JOURNAL OF ENDODONTICS (2015).