Functional characterization of various algal carotenoid ketolases   reveals that ketolating zeaxanthin efficiently is essential for high   production of astaxanthin in transgenic Arabidopsis

doi:10.1093/jxb/err070

CORC > 北京大学 > 工学院

	Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis
	Zhong, Yu-Juan ; Huang, Jun-Chao ; Liu, Jin ; Li, Yin ; Jiang, Yue ; Xu, Zeng-Fu ; Sandmann, Gerhard ; Chen, Feng
刊名	journal of experimental botany
	2011
关键词	Arabidopsis thaliana astaxanthin beta-carotene ketolase carotenoid Haematococcus pluvialis KETOCAROTENOID BIOSYNTHESIS HAEMATOCOCCUS-PLUVIALIS CHLAMYDOMONAS-REINHARDTII PHOTOOXIDATIVE STRESS VITAMIN-E PATHWAY PLANTS FLOWERS GENE EXPRESSION
DOI	10.1093/jxb/err070
英文摘要	Extending the carotenoid pathway to astaxanthin in plants is of scientific and industrial interest. However, expression of a microbial beta-carotene ketolase (BKT) that catalyses the formation of ketocarotenoids in transgenic plants typically results in low levels of astaxanthin. The low efficiency of BKTs in ketolating zeaxanthin to astaxanthin is proposed to be the major limitation for astaxanthin accumulation in engineered plants. To verify this hypothesis, several algal BKTs were functionally characterized using an Escherichia coli system and three BKTs were identified, with high (up to 85%), moderate (similar to 38%), and low (similar to 1%) conversion rate from zeaxanthin to astaxanthin from Chlamydomonas reinhardtii (CrBKT), Chlorella zofingiensis (CzBKT), and Haematococcus pluvialis (HpBKT3), respectively. Transgenic Arabidopsis thaliana expressing the CrBKT developed orange leaves which accumulated astaxanthin up to 2 mg g(-1) dry weight with a 1.8-fold increase in total carotenoids. In contrast, the expression of CzBKT resulted in much lower astaxanthin content (0.24 mg g(-1) dry weight), whereas HpBKT3 was unable to mediate synthesis of astaxanthin in A. thaliana. The none-native astaxanthin was found mostly in a free form integrated into the light-harvesting complexes of photosystem II in young leaves but in esterified forms in senescent leaves. The alteration of carotenoids did not affect chlorophyll content, plant growth, or development significantly. The astaxanthin-producing plants were more tolerant to high light as shown by reduced lipid peroxidation. This study advances a decisive step towards the utilization of plants for the production of high-value astaxanthin.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292557000032&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Plant Sciences; SCI(E); 23; ARTICLE; 10; 3659-3669; 62
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/394752]
专题	工学院
推荐引用方式 GB/T 7714	Zhong, Yu-Juan,Huang, Jun-Chao,Liu, Jin,et al. Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis[J]. journal of experimental botany,2011.
APA	Zhong, Yu-Juan.,Huang, Jun-Chao.,Liu, Jin.,Li, Yin.,Jiang, Yue.,...&Chen, Feng.(2011).Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis.journal of experimental botany.
MLA	Zhong, Yu-Juan,et al."Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis".journal of experimental botany (2011).