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	Two-Color CW STED Nanoscopy
	Chen, Xuanze ; Liu, Yujia ; Yang, Xusan ; Wang, Tingting ; Alonas, Eric ; Santangelo, Philip J. ; Ren, Qiushi ; Xi, Peng
	2013
关键词	Two-color CW STED super-resolution colocalization CONFOCAL MICROSCOPY FLUORESCENCE MICROSCOPY RESOLUTION BEAMS LIMIT
DOI	10.1117/12.2001178
英文摘要	Fluorescent microscopy has become an essential tool to study biological molecules, pathways and events in living cells, tissues and animals. Meanwhile even the most advanced confocal microscopy can only yield optical resolution approaching Abbe diffraction limit of similar to 200 nm. This is still larger than many subcellular structures, which are too small to be resolved in detail. These limitations have driven the development of super-resolution optical imaging methodologies over the past decade. In stimulated emission depletion (STED) microscopy, the excitation focus is overlapped by an intense doughnut-shaped spot to instantly de-excite markers from their fluorescent state to the ground state by stimulated emission. This effectively eliminates the periphery of the Point Spread Function (PSF), resulting in a narrower focal region, or super-resolution. Scanning a sharpened spot through the specimen renders images with sub-diffraction resolution. Multi-color STED imaging can present important structural and functional information for protein-protein interaction. In this work, we presented a two-color, synchronization-free STED microscopy with a Ti:Sapphire oscillator. The excitation wavelengths were 532nm and 635nm, respectively. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. Human respiratory syncytial virus (hRSV) proteins were imaged with our two-color CW STED for co-localization analysis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000321741600023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Optics; Radiology, Nuclear Medicine & Medical Imaging; Spectroscopy; EI; CPCI-S(ISTP); 1
语种	英语
内容类型	会议论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/316389]
专题	工学院
推荐引用方式 GB/T 7714	Chen, Xuanze,Liu, Yujia,Yang, Xusan,et al. Two-Color CW STED Nanoscopy[C]. 见:.

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