Barcoded Microchips for Biomolecular Assays

doi:10.1021/ac5032379

CORC > 北京大学 > 工学院

	Barcoded Microchips for Biomolecular Assays
	Zhang, Yi ; Sun, Jiashu ; Zou, Yu ; Chen, Wenwen ; Zhang, Wei ; Xi, Jianzhong Jeff ; Jiang, Xingyu
刊名	analytical chemistry
	2015
关键词	PROTEIN-DETECTION NANODISK CODES PARTICLES DNA FLUORESCENCE TARGETS ARRAYS CELL
DOI	10.1021/ac5032379
英文摘要	Multiplexed assay of analytes is of great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode and decode may realize this goal in a straightforward and consistent manner. We present here a microfluidic barcoded chip containing several sets of microchannels with different widths, imitating the commonly used barcode. A single barcoded microchip can carry out tens of individual protein/nucleic acid assays (encode) and immediately yield all assay results by a portable barcode reader or a smartphone (decode). The applicability of a barcoded microchip is demonstrated by human immunodeficiency virus (HIV) immunoassays for simultaneous detection of three targets (anti-gp41 antibody, anti-gp120 antibody, and anti-gp36 antibody) from six human serum samples. We can also determine seven pathogen-specific oligonucleotides by a single chip containing both positive and negative controls.; Chemistry, Analytical; SCI(E); EI; 0; ARTICLE; sunjs@nanoctr.cn; zhangw@nanoctr.cn; xingyujiang@nanoctr.cn; 2; 900-906; 87
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315729]
专题	工学院
推荐引用方式 GB/T 7714	Zhang, Yi,Sun, Jiashu,Zou, Yu,et al. Barcoded Microchips for Biomolecular Assays[J]. analytical chemistry,2015.
APA	Zhang, Yi.,Sun, Jiashu.,Zou, Yu.,Chen, Wenwen.,Zhang, Wei.,...&Jiang, Xingyu.(2015).Barcoded Microchips for Biomolecular Assays.analytical chemistry.
MLA	Zhang, Yi,et al."Barcoded Microchips for Biomolecular Assays".analytical chemistry (2015).