MiR-301a Mediates the Effect of IL-6 on the AKT/GSK Pathway and Hepatic Glycogenesis by Regulating PTEN Expression

doi:10.1159/000373962

CORC > 北京大学 > 工学院

	MiR-301a Mediates the Effect of IL-6 on the AKT/GSK Pathway and Hepatic Glycogenesis by Regulating PTEN Expression
	Dou Lin ; Wang Shuyue ; Sui Xiaofang ; Meng Xiangyu ; Shen Tao ; Huang Xiuqing ; Guo Jun ; Fang Weiwei ; Man Yong ; Xi Jianzhong ; Li Jian
刊名	cellular physiology and biochemistry international journal of experimental cellular physiology biochemistry and pharmacology
	2015
DOI	10.1159/000373962
英文摘要	IL-6 has been implicated in the pathogenesis of insulin resistance. MiR-301a plays an important role in various biological and pathological processes, including cellular development and differentiation, inflammation, apoptosis and cancer. However, whether miR-301a mediates IL-6-induced insulin resistance in hepatocytes remains unknown.The activation of AKT/GSK pathway and the level of glycogenesis were examed in NCTC 1469 cells transfected miR-301a mimics and inhibitor. Using computational miRNA target prediction database, PTEN was a target of miR-301a. The effect of miR-301a on PTEN expression was evaluated using Luciferase assay and western blot. A PTEN-specific siRNA was used to further determine the effect of PTEN on IL-6-induced insulin resistance.In vivo and in vitro treatment with IL-6 was led to down-regulation of miR-301a, accompanied by impairment of theAKT/GSK pathway and glycogenesis. Importantly, over-expression of miR-301a rescued IL-6-induced decreased activation of the AKT/GSK pathway and hepatic glycogenesis. In contrast, down-regulation of miR-301a induced impaired phosphorylation of AKT and GSK, accompanied by reduced glycogenesis in hepatocytes. Moreover, our results indicate that suppression of PTEN, a target of miR-301a, diminished the effect of IL-6 on the AKT/GSK pathway and hepatic glycogenesis.We present novel evidence of the contribution of miR-301a to IL-6-induced insulin resistance by direct regulation of PTEN expression. ? 2015 S. Karger AG, Basel.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352457400014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); PubMed; 6; ARTICLE; lijian@bjhmoh.cn; jzxi@pku.edu.cn; 4; 1413-24; 35
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315608]
专题	工学院
推荐引用方式 GB/T 7714	Dou Lin,Wang Shuyue,Sui Xiaofang,et al. MiR-301a Mediates the Effect of IL-6 on the AKT/GSK Pathway and Hepatic Glycogenesis by Regulating PTEN Expression[J]. cellular physiology and biochemistry international journal of experimental cellular physiology biochemistry and pharmacology,2015.
APA	Dou Lin.,Wang Shuyue.,Sui Xiaofang.,Meng Xiangyu.,Shen Tao.,...&Li Jian.(2015).MiR-301a Mediates the Effect of IL-6 on the AKT/GSK Pathway and Hepatic Glycogenesis by Regulating PTEN Expression.cellular physiology and biochemistry international journal of experimental cellular physiology biochemistry and pharmacology.
MLA	Dou Lin,et al."MiR-301a Mediates the Effect of IL-6 on the AKT/GSK Pathway and Hepatic Glycogenesis by Regulating PTEN Expression".cellular physiology and biochemistry international journal of experimental cellular physiology biochemistry and pharmacology (2015).