Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of   HeLa cells

doi:10.1016/j.jprot.2011.08.014

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	Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells
	Xu, Yang ; Ching, Yick-Pang ; Zhou, Yuan ; Chiu, Jen-Fu ; Chen, Feng ; He, Qing-Yu
刊名	journal of proteomics
	2011
关键词	Cytoskeleton Cytotoxicity ER Mitochondria Tubeimoside-1 MITOCHONDRIAL PERMEABILITY TRANSITION ENDOPLASMIC-RETICULUM STRESS DNA-DAMAGE CHECKPOINT KINASE-ACTIVITY CYCLE ARREST TRITERPENOID SAPONINS CARCINOMA-CELLS BINDING-SITE APOPTOSIS PHOSPHORYLATION
DOI	10.1016/j.jprot.2011.08.014
英文摘要	The Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae) is a Chinese herb with anticancer potential. Its main active component tubeimoside-1 (TBMS1), a triterpenoid saponin, was previously proved as a potent anticancer chemotherapeutic agent; however, the molecular basis for its activities is still elusive. In the present study, subcellular proteomic study in the cytoplasm and membrane protein fractions extracted from HeLa cells revealed that proteins act as mediators of ROS generation and Ca(2+) regulation were substantially altered in expression upon TBMS1 stimuli. We also found that TBMS1 induced cell cycle arrest at G2/M phase accompanied by a decrease in GO/G1 phase in HeLa cells. Further biochemical studies showed that TBMS1 inhibited the levels of cyclinB1, Cdc2 and Cdc25C, but enhanced Chk2 phosphorylation. In addition, the cytoplasm sequestration of Cdc25C, Cip1/p21 induction and tubulin dyspolymerization also contributed to the TBMS1-mediated cell cycle arrest on the G2/M phase. (C) 2011 Elsevier B.V. All rights reserved.; Biochemical Research Methods; SCI(E); 0; ARTICLE; 2; 491-501; 75
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/236859]
专题	工学院
推荐引用方式 GB/T 7714	Xu, Yang,Ching, Yick-Pang,Zhou, Yuan,et al. Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells[J]. journal of proteomics,2011.
APA	Xu, Yang,Ching, Yick-Pang,Zhou, Yuan,Chiu, Jen-Fu,Chen, Feng,&He, Qing-Yu.(2011).Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells.journal of proteomics.
MLA	Xu, Yang,et al."Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells".journal of proteomics (2011).