Molecular mechanisms of the coordination between astaxanthin and fatty   acid biosynthesis in Haematococcus pluvialis (Chlorophyceae)

doi:10.1111/tpj.12713

CORC > 北京大学 > 工学院

	Molecular mechanisms of the coordination between astaxanthin and fatty acid biosynthesis in Haematococcus pluvialis (Chlorophyceae)
	Chen, Guanqun ; Wang, Baobei ; Han, Danxiang ; Sommerfeld, Milton ; Lu, Yinghua ; Chen, Feng ; Hu, Qiang
刊名	plant journal
	2015
关键词	Haematococcus pluvialis fatty acid biosynthesis carotenoid microalgae gene transcription metabolic coordination acyl CoA diacylglycerol acyltransferase CAROTENOID BIOSYNTHESIS OLEIC-ACID TRIACYLGLYCEROL ACCUMULATION OXIDATIVE STRESS GENES CAROTENOGENESIS ENZYMES ARABIDOPSIS CARBOXYLASE INHIBITION
DOI	10.1111/tpj.12713
英文摘要	Astaxanthin, a red ketocarotenoid with strong antioxidant activity and high commercial value, possesses important physiological functions in astaxanthin-producing microalgae. The green microalga Haematococcus pluvialis accumulates up to 4% fatty acid-esterified astaxanthin (by dry weight), and is used as a model species for exploring astaxanthin biosynthesis in unicellular photosynthetic organisms. Although coordination of astaxanthin and fatty acid biosynthesis in a stoichiometric fashion was observed in H. pluvialis, the interaction mechanism is unclear. Here we dissected the molecular mechanism underlying coordination between the two pathways in H. pluvialis. Our results eliminated possible coordination of this inter-dependence at the transcriptional level, and showed that this interaction was feedback-coordinated at the metabolite level. In vivo and in vitro experiments indicated that astaxanthin esterification drove the formation and accumulation of astaxanthin. We further showed that both free astaxanthin biosynthesis and esterification occurred in the endoplasmic reticulum, and that certain diacylglycerol acyltransferases may be the candidate enzymes catalyzing astaxanthin esterification. A model of astaxanthin biosynthesis in H. pluvialis was subsequently proposed. These findings provide further insights into astaxanthin biosynthesis in H. pluvialis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000346918400008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Plant Sciences; SCI(E); EI; 4; ARTICLE; huqiang@asu.edu; 1; 95-107; 81
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/206697]
专题	工学院
推荐引用方式 GB/T 7714	Chen, Guanqun,Wang, Baobei,Han, Danxiang,et al. Molecular mechanisms of the coordination between astaxanthin and fatty acid biosynthesis in Haematococcus pluvialis (Chlorophyceae)[J]. plant journal,2015.
APA	Chen, Guanqun.,Wang, Baobei.,Han, Danxiang.,Sommerfeld, Milton.,Lu, Yinghua.,...&Hu, Qiang.(2015).Molecular mechanisms of the coordination between astaxanthin and fatty acid biosynthesis in Haematococcus pluvialis (Chlorophyceae).plant journal.
MLA	Chen, Guanqun,et al."Molecular mechanisms of the coordination between astaxanthin and fatty acid biosynthesis in Haematococcus pluvialis (Chlorophyceae)".plant journal (2015).