Directing chondrogenic differentiation of mesenchymal stem cells with a   solid-supported chitosan thermogel for cartilage tissue engineering

doi:10.1088/1748-6041/9/3/035008

CORC > 北京大学 > 工学院

	Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering
	Huang, Hongjie ; Zhang, Xin ; Hu, Xiaoqing ; Dai, Linghui ; Zhu, Jingxian ; Man, Zhentao ; Chen, Haifeng ; Zhou, Chunyan ; Ao, Yingfang
刊名	biomedical materials
	2014
关键词	mesenchymal stem cell thermogel demineralized bone matrix chondrogenesis cartilage tissue engineering MARROW STROMAL CELLS CORTICAL BONE-MATRIX IN-VIVO ARTICULAR-CARTILAGE MECHANICAL-PROPERTIES DEFECT REPAIR HYDROGELS VITRO THERAPY MICROFRACTURE
DOI	10.1088/1748-6041/9/3/035008
英文摘要	Hydrogels are attractive for cartilage tissue engineering because of their high plasticity and similarity with the native cartilage matrix. However, one critical drawback of hydrogels for osteochondral repair is their inadequate mechanical strength. To address this limitation, we constructed a solid-supported thermogel comprising a chitosan hydrogel system and demineralized bone matrix. Scanning electron microscopy, the equilibrium scanning ratio, the biodegradation rate, biomechanical tests, biochemical assays, metabolic activity tests, immunostaining and cartilage-specific gene expression analysis were used to evaluate the solid-supported thermogel. Compared with pure hydrogel or demineralized matrix, the hybrid biomaterial showed superior porosity, equilibrium swelling and degradation rate. The hybrid scaffolds exhibited an increased mechanical strength: 75% and 30% higher compared with pure hydrogels and demineralized matrix, respectively. After three days culture, bone-derived mesenchymal stem cells (BMSCs) maintained viability above 90% in all three materials; however, the cell retention of the hybrid scaffolds was more efficient and uniform than the other materials. Matrix production and chondrogenic differentiation of BMSCs in the hybrid scaffolds were superior to its precursors, based on glycosaminoglycan quantification and hyaline cartilage marker expression after three weeks in culture. Its easy preparation, favourable biophysical properties and chondrogenic capacity indicated that this solid-supported thermogel could be an attractive biomaterial framework for cartilage tissue engineering.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336738000009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; PubMed; 6; ARTICLE; chunyanzhou@bjmu.edu.cn; yingfang.ao@gmail.com; 3; 035008; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/161337]
专题	工学院
推荐引用方式 GB/T 7714	Huang, Hongjie,Zhang, Xin,Hu, Xiaoqing,et al. Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering[J]. biomedical materials,2014.
APA	Huang, Hongjie.,Zhang, Xin.,Hu, Xiaoqing.,Dai, Linghui.,Zhu, Jingxian.,...&Ao, Yingfang.(2014).Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering.biomedical materials.
MLA	Huang, Hongjie,et al."Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering".biomedical materials (2014).