Guided assembly of endothelial cells on hydrogel matrices patterned with   microgrooves: a basic model for microvessel engineering

doi:10.1039/c2sm27126e

CORC > 北京大学 > 工学院

	Guided assembly of endothelial cells on hydrogel matrices patterned with microgrooves: a basic model for microvessel engineering
	Jiang, Li-Yang ; Luo, Ying
刊名	软性材料
	2013
关键词	GLYCOL) DIACRYLATE HYDROGELS IN-VITRO HYALURONIC-ACID EXTRACELLULAR-MATRIX TISSUE ANGIOGENESIS DIFFERENTIATION VASCULARIZATION NETWORKS FATE
DOI	10.1039/c2sm27126e
英文摘要	Engineering microvessel structures with spatial control has important implications for tissue engineering and regenerative medicine. We demonstrate a facile approach to integrate structural and biochemical patterns on biodegradable hydrogel matrices via combining soft-lithographic and micromolding techniques to enable self-assembly of endothelial cells toward vessel morphogenesis. Human umbilical cord vein endothelial cells (HUVECs), with or without stromal fibroblasts, were cultured on matrices made of hyaluronic acid-dextran or agarose hydrogels patterned with microgrooves containing entrapped collagen. It was found that only with appropriate microgroove geometry would the patterned matrices induce the aggregation and coalescence of endothelial cells to form microvessel-like structures. On the basis of the monoculture model, a co-culture configuration mimicking the natural vessel morphology was also established on the hyaluronic acid-dextran matrix, which contained double layered cellular assemblies through recruitment of fibroblasts to the surface of the pre-assembled endothelial cords. This study shows that the hydrogel matrices patterned with microgrooves provide a new and versatile system to enable the morphogenetic assembling of cells and warrant further studies for engineering microvessel tissues.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000312553900013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Physical; Materials Science, Multidisciplinary; Physics, Multidisciplinary; Polymer Science; SCI(E); EI; 6; ARTICLE; 4; 1113-1121; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154552]
专题	工学院
推荐引用方式 GB/T 7714	Jiang, Li-Yang,Luo, Ying. Guided assembly of endothelial cells on hydrogel matrices patterned with microgrooves: a basic model for microvessel engineering[J]. 软性材料,2013.
APA	Jiang, Li-Yang,&Luo, Ying.(2013).Guided assembly of endothelial cells on hydrogel matrices patterned with microgrooves: a basic model for microvessel engineering.软性材料.
MLA	Jiang, Li-Yang,et al."Guided assembly of endothelial cells on hydrogel matrices patterned with microgrooves: a basic model for microvessel engineering".软性材料 (2013).