Real-Time Monitoring of Nitric Oxide at Single-Cell Level with   Porphyrin-Functionalized Graphene Field-Effect Transistor Biosensor

doi:10.1021/acs.analchem.6b03208

CORC > 北京大学 > 信息科学技术学院

	Real-Time Monitoring of Nitric Oxide at Single-Cell Level with Porphyrin-Functionalized Graphene Field-Effect Transistor Biosensor
	Xie, Hui ; Li, Yu-Tao ; Lei, Yong-Min ; Liu, Yan-Ling ; Xiao, Meng-Meng ; Gao, Chuan ; Pang, Dai-Wen ; Huang, Wei-Hua ; Zhang, Zhi-Yong ; Zhang, Guo-Jun
刊名	ANALYTICAL CHEMISTRY
	2016
关键词	CHEMICAL-ANALYSIS SENSITIVITY NANOCOMPOSITE COMPLEXES NANOWIRE SYSTEM SHEETS AGENT FILMS NO
DOI	10.1021/acs.analchem.6b03208
英文摘要	An ultrasensitive and highly efficient assay for real-time monitoring of nitric oxide (NO) at single-cell level based on a reduced graphene oxide (RGO) and iron porphyrin-functionalized graphene (FGPCs) field-effect transistor (FET) biosensor is reported. A layer-to layer assembly of RGO and FGPCs on a prefabricated FET sensor surface through pi-pi stacking interaction allowed superior electrical conductivity caused by RGO, and highly catalytic specificity induced by metalloporphyrin, ensuring the ultrasensitive and highly specific detection of NO. The results demonstrated that the RGO/FGPCs FET biosensor was capable of real-time monitoring of NO in the range from 1 pM to 100 nM with the limit of detection as low as 1 pM in phosphate-buffered saline (PBS) and 10 pM in the cell medium,. respectively. Moreover, the developed biosensor could be used for real-time monitoring of NO released from human umbilical vein endothelial cells (HUVECs) at single-cell level. Along with its miniaturized sizes, ultrasensitive characteristics, and fast response, the FET biosensor is promising as a new platform for potential biological and diagnostic applications.; National Natural Science Foundation of China [21275040, 21475034, 61390504]; SCI(E); PubMed; ARTICLE; whhuang@whu.edu.cn; zyzhang@pku.edu.cn; zhanggj@hbtcm.edu.cn; 22; 11115-11122; 88
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457117]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Xie, Hui,Li, Yu-Tao,Lei, Yong-Min,et al. Real-Time Monitoring of Nitric Oxide at Single-Cell Level with Porphyrin-Functionalized Graphene Field-Effect Transistor Biosensor[J]. ANALYTICAL CHEMISTRY,2016.
APA	Xie, Hui.,Li, Yu-Tao.,Lei, Yong-Min.,Liu, Yan-Ling.,Xiao, Meng-Meng.,...&Zhang, Guo-Jun.(2016).Real-Time Monitoring of Nitric Oxide at Single-Cell Level with Porphyrin-Functionalized Graphene Field-Effect Transistor Biosensor.ANALYTICAL CHEMISTRY.
MLA	Xie, Hui,et al."Real-Time Monitoring of Nitric Oxide at Single-Cell Level with Porphyrin-Functionalized Graphene Field-Effect Transistor Biosensor".ANALYTICAL CHEMISTRY (2016).