High specific surface gold electrode on polystyrene substrate:   Characterization and application as DNA biosensor

doi:10.1016/j.talanta.2016.02.006

CORC > 北京大学 > 信息科学技术学院

	High specific surface gold electrode on polystyrene substrate: Characterization and application as DNA biosensor
	Yang, Zhiliu ; Liu, Yichen ; Lu, Wei ; Yuan, Qingpan ; Wang, Wei ; Pu, Qiaosheng ; Yao, Bo
刊名	TALANTA
	2016
关键词	DNA Biosensing Gold electrode Polystyrene Electrochemical detection ROLLING CIRCLE AMPLIFICATION ESCHERICHIA-COLI O157-H7 ELECTROCHEMICAL DETECTION SIGNAL AMPLIFICATION NUCLEIC-ACIDS HYBRIDIZATION NANOPARTICLES PLATFORM SENSOR MICROELECTRODE
DOI	10.1016/j.talanta.2016.02.006
英文摘要	In the past decades, many efforts have been made to improve the sensitivity and specificity of electrochemical DNA biosensors. However, it is still strongly required to develop disposable and reliable DNA biosensors for wide and practical application. In this article, we reported superior electrochemical properties of an integrated plastic-gold electrode (PGE) fabricated in-house by chemical plating on polystyrene substrate. PGEs were found having extremely high capacity of DNA immobilization compared with gold electrodes fabricated by standard sputtering based photolithography. Unique nano structured surface was observed on PGEs through morphology techniques, which would to some extend give an explanation to higher capacity of DNA immobilization on PGEs. A probable mechanism of carboxylic acid produced on polystyrene substrate after exposure to UV irradiation was proposed and discussed for the first time. This biosensor was applied to detection and manipulate of DNA hybridization. Detection limit of 7.2 x 10(-11) M and 1-500 nM of linearity range was obtained. (C) 2016 Published by Elsevier B.V.; National Natural Science Foundation of China [21375119, 21175062, 81471750]; Major National Science and Technology Programs of China [2013ZX09507005]; Fundamental Research Funds for the Central Universities [2015QNA3009]; SCI(E); EI; PubMed; ARTICLE; puqs@lzu.edu.cn; yaobo08@zju.edu.cn; 301-307; 152
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434451]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Yang, Zhiliu,Liu, Yichen,Lu, Wei,et al. High specific surface gold electrode on polystyrene substrate: Characterization and application as DNA biosensor[J]. TALANTA,2016.
APA	Yang, Zhiliu.,Liu, Yichen.,Lu, Wei.,Yuan, Qingpan.,Wang, Wei.,...&Yao, Bo.(2016).High specific surface gold electrode on polystyrene substrate: Characterization and application as DNA biosensor.TALANTA.
MLA	Yang, Zhiliu,et al."High specific surface gold electrode on polystyrene substrate: Characterization and application as DNA biosensor".TALANTA (2016).