A Pliable Electroporation Patch (ep-Patch) for Efficient Delivery of   Nucleic Acid Molecules into Animal Tissues with Irregular Surface Shapes

doi:10.1038/srep07618

CORC > 北京大学 > 信息科学技术学院

	A Pliable Electroporation Patch (ep-Patch) for Efficient Delivery of Nucleic Acid Molecules into Animal Tissues with Irregular Surface Shapes
	Wei, Zewen ; Huang, Yuanyu ; Zhao, Deyao ; Hu, Zhiyuan ; Li, Zhihong ; Liang, Zicai
刊名	scientific reports
	2015
关键词	LEBERS CONGENITAL AMAUROSIS GENE-THERAPY IN-VIVO SIRNA DELIVERY DNA OPTIMIZATION PROGRESS RNAI NANOPARTICLES THERAPEUTICS
DOI	10.1038/srep07618
英文摘要	Delivery of nucleic acids into animal tissues by electroporation is an appealing approach for various types of gene therapy, but efficiency of existing methodsis not satisfactory. Here we present the validation of novel electroporation patch (ep-Patch) for efficient delivery of DNA and siRNA into mouse tissues. Using micromachining technology, closely spaced gold electrodes were made on the pliable parylene substrate to form a patch-like electroporation metrics. It enabled large coverage of the target tissues and close surface contact between the tissues and electrodes, thus providing a uniform electric field to deliver nucleic acids into tissues, even beneath intact skin. Using this ep-Patch for efficiently delivery of both DNA and siRNA, non-invasive electroporation of healthy mouse muscle tissue was successfully achieved. Delivery of these nucleic acids was performed to intact tumors with satisfactory results. Silencing of tumor genes using the ep-Patch was also demonstrated on mice. This pliable electroporation patch method constitutes a novel way of in vivo delivery of siRNA and DNA to certain tissues or organs to circumvent the disadvantages of existing methodologies for in vivo delivery of nucleic acid molecules.; Multidisciplinary Sciences; SCI(E); PubMed; 0; ARTICLE; huzy@nanoctr.cn; zhhli@ime.pku.edu.cn; liangz@pku.edu.cn; 7618; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/341711]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Wei, Zewen,Huang, Yuanyu,Zhao, Deyao,et al. A Pliable Electroporation Patch (ep-Patch) for Efficient Delivery of Nucleic Acid Molecules into Animal Tissues with Irregular Surface Shapes[J]. scientific reports,2015.
APA	Wei, Zewen,Huang, Yuanyu,Zhao, Deyao,Hu, Zhiyuan,Li, Zhihong,&Liang, Zicai.(2015).A Pliable Electroporation Patch (ep-Patch) for Efficient Delivery of Nucleic Acid Molecules into Animal Tissues with Irregular Surface Shapes.scientific reports.
MLA	Wei, Zewen,et al."A Pliable Electroporation Patch (ep-Patch) for Efficient Delivery of Nucleic Acid Molecules into Animal Tissues with Irregular Surface Shapes".scientific reports (2015).