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	A FLOW-THROUGH ELECTROPORATION CHIP INTEGRATED WITH VIABLE CELL SORTING BASED ON DIELECTROPHORESIS
	Li, Xueming ; Wei, Zewen ; Zhao, Deyao ; Yan, Hao ; Liang, Zicai ; Li, Zhihong
	2012
英文摘要	Flow-through electroporation on a chip is a promising technique to introduce molecules into cells in applications of biological research, drug delivery and gene therapy. During the electroporation process, the electrical field also causes large amount of non-viable cells, which significantly influence the following biological procedure. Off-chip separation of the variable cells increases experimental complexity dramatically and results in further cell death. In this paper, we have developed a flow-through electroporation chip, in which dielectrophoresis (DEP) is employed to sort the viable cells and non-viable cells on chip. For the standard expression cell line HEK-293a (Human embryonic kidney cells), the ratio of the viable cells in the sorted sample was increased to 90% from 20% in the as-electroporated sample.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000312912800250&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Electrical & Electronic; Nanoscience & Nanotechnology; EI; CPCI-S(ISTP); 0
语种	英语
DOI标识	10.1109/MEMSYS.2012.6170353
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/292838]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Li, Xueming,Wei, Zewen,Zhao, Deyao,et al. A FLOW-THROUGH ELECTROPORATION CHIP INTEGRATED WITH VIABLE CELL SORTING BASED ON DIELECTROPHORESIS. 2012-01-01.

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