A flexible microneedle array as low-voltage electroporation electrodes   for in vivo DNA and siRNA delivery

doi:10.1039/c4lc00800f

CORC > 北京大学 > 信息科学技术学院

	A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery
	Wei, Zewen ; Zheng, Shuquan ; Wang, Renxin ; Bu, Xiangli ; Ma, Huailei ; Wu, Yidi ; Zhu, Ling ; Hu, Zhiyuan ; Liang, Zicai ; Li, Zhihong
刊名	芯片实验室
	2014
关键词	GENE-THERAPY THERAPEUTICS EXPRESSION VACCINES PROGRESS PLASMID MUSCLE DRUG
DOI	10.1039/c4lc00800f
英文摘要	In vivo electroporation is an appealing method to deliver nucleic acid into living tissues, but the clinical application of such a method was limited due to severe tissue damage and poor coverage of the tissue surface. Here we present the validation of a novel flexible microneedle array electrode (MNAE) chip, in which the microneedle array and the flexible substrate are integrated together to simultaneously facilitate low-voltage electroporation and accomplish good coverage of the tissue surface. The efficient delivery of both DNA and siRNA was demonstrated on mice. Upon penetrating the high-resistance stratum corneum, the electroporation voltage was reduced to about 35 V. which was generally recognized safe for humans. Also, a pathological analysis of the microneedle-electroporated tissues was carried out to thoroughly assess the skin damage, which is an important consideration in pre-clinical studies of electroporation devices. This MNAE constitutes a novel way of in vivo delivery of siRNA and DNA to certain tissues or organs with satisfactory efficiency and good adaptation to the tissue surface profile as well as minimum tissue damage, thus avoiding the disadvantages of existing electroporation methods.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000342866400023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; SCI(E); 4; ARTICLE; Huzy@nanoctr.cn; Liangz@pku.edu.cn; Zhhli@ime.pku.edu.cn; 20; 4093-4102; 14
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/209926]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Wei, Zewen,Zheng, Shuquan,Wang, Renxin,et al. A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery[J]. 芯片实验室,2014.
APA	Wei, Zewen.,Zheng, Shuquan.,Wang, Renxin.,Bu, Xiangli.,Ma, Huailei.,...&Li, Zhihong.(2014).A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery.芯片实验室.
MLA	Wei, Zewen,et al."A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery".芯片实验室 (2014).