Silicon inhibition effects on the polymerase chain reaction: A real-time   detection approach

doi:10.1002/jbm.a.30627

CORC > 北京大学 > 信息科学技术学院

	Silicon inhibition effects on the polymerase chain reaction: A real-time detection approach
	Wang, W ; Wang, HB ; Li, ZX ; Guo, ZY
刊名	journal of biomedical materials research part a
	2006
关键词	real-time PCR inhibition effects silicon nanoparticles RAY PHOTOELECTRON-SPECTROSCOPY DNA AMPLIFICATION SURFACE PASSIVATION OXIDIZED SILICON GLASS CHIPS PCR OPTIMIZATION MICROSTRUCTURES MICROCHAMBERS EFFICIENT
DOI	10.1002/jbm.a.30627
英文摘要	In the miniaturization of biochemical analysis systems, biocompatibility of the microfabricated material is a key feature to be considered. A clear insight into interactions between biological reagents and microchip materials will help to build more robust functional bio-microelectro-mechanical systems (BioMEMS). In the present work, a real-time polymerase chain reaction (PCR) assay was used to study the inhibition effects of silicon and native silicon oxide particles on Hepatitis B Virus (HBV) DNA PCR amplification. Silicon nanoparticles with different surface oxides were added into the PCR mixture to activate possible interactions between the silicon-related materials and the PCR reagents. Ratios of silicon nanoparticle surface area to PCR mixture volume (surface to volume ratio) varied from 4.7 to 235.5 mm(2)/mu L. Using high speed centrifugation, the nanoparticles were pelleted to tube inner surfaces. Supernatant extracts were then used in subsequent PCR experiments. To test whether silicon materials participated in amplifications directly, in some cases, entire PCR mixture containing silicon nanoparticles were used in amplification. Fluorescence histories of PCR amplifications indicated that with the increase in surface to volume ratio, amplification efficiency decreased considerably, and within the studied ranges, the higher the particle surface oxidation, the stronger the silicon inhibition effects on PCR. Adsorption of Taq polymerise (not nucleic acid) on the silicon-related material surface was the primary cause of the inhibition phenomena and silicon did not participate in the amplification process directly. (c) 2005 Wiley Periodicals, Inc.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000235945000004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; 17; ARTICLE; 1; 28-34; 77A
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/153030]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Wang, W,Wang, HB,Li, ZX,et al. Silicon inhibition effects on the polymerase chain reaction: A real-time detection approach[J]. journal of biomedical materials research part a,2006.
APA	Wang, W,Wang, HB,Li, ZX,&Guo, ZY.(2006).Silicon inhibition effects on the polymerase chain reaction: A real-time detection approach.journal of biomedical materials research part a.
MLA	Wang, W,et al."Silicon inhibition effects on the polymerase chain reaction: A real-time detection approach".journal of biomedical materials research part a (2006).