Reaction mechanism of dicofol removal by cellulase

doi:10.1016/j.jes.2015.03.015

CORC > 北京大学 > 环境科学与工程学院

	Reaction mechanism of dicofol removal by cellulase
	Wang Ziyuan ; Yang Ting ; Zhai Zihan ; Zhang Boya ; Zhang Jianbo
刊名	Journal of Environmental Sciences
	2015
关键词	Dicofol Cellulase Endo-glucanases activity Oxidative degradation OH free radical BIODEGRADATION KINETICS CHILDHOOD-CANCER ROT FUNGI PESTICIDES SOIL DDT DEGRADATION ESTROGEN RECEPTOR ENZYME
DOI	10.1016/j.jes.2015.03.015
英文摘要	It remains unclear whether dicofol should be defined as a persistent organic pollutant. Its environmental persistence has gained attention. This study focused on its degradation by cellulase. Cellulase was separated using a gel chromatogram, and its degradation activity towards dicofol involved its endoglucanase activity. By analyzing the kinetic parameters of cellulase reacting with mixed substrates, it was shown that cellulase reacted on dicofol and carboxyl methyl cellulose through two different active centers. Thus, the degradation of dicofol was shown to be an oxidative process by cellulase. Next, by comparing the impacts of tert-butyl alcohol (a typical OH free-radical inhibitor) on the removal efficiencies of dicofol under both cellulase and Fenton reagent systems, it was shown that the removal of dicofol was initiated by OH free radicals produced by cellulase. Finally, 4,4'-dichlorodibenzophenone and chloride were detected using gas chromatography mass spectrometry and ion chromatography analysis, which supported our hypothesis. The reaction mechanism was analyzed and involved an attack by OH free radicals at the orthocarbon of dicofol, resulting in the degradation product 4,4'-dichloro-dibenzophenone.; supported by the special fund provided by the State Key Joint Laboratory of Environmental Simulation and Pollution Control (Peking University),the Special Research Funding for Public Benefit Industries from National Ministry of Environmental Protection; SCI(E); EI; PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); jbzhang@pku.edu.cn; 10; 36, 22-28; 36
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/446974]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Wang Ziyuan,Yang Ting,Zhai Zihan,et al. Reaction mechanism of dicofol removal by cellulase[J]. Journal of Environmental Sciences,2015.
APA	Wang Ziyuan,Yang Ting,Zhai Zihan,Zhang Boya,&Zhang Jianbo.(2015).Reaction mechanism of dicofol removal by cellulase.Journal of Environmental Sciences.
MLA	Wang Ziyuan,et al."Reaction mechanism of dicofol removal by cellulase".Journal of Environmental Sciences (2015).